Incidence of Faecal coliforms Isolated From Different Foods Sold Locally in Nsukka, Enugu State
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Abstract
Sixty-six (66) food products and ten water samples were collected from food vendors and restaurants and examined for bacteriological quality using both the most probable number (MPN) technique and the plate count of Escherichia coli on Eosin Ethylene Blue (EMB) and lactose fermenters on MacConkey agar. All results obtained were analyzed using ANOVA and Duncan’s multiple range test. Out of the 66
food samples, 55.6% were positive for Escherichia coli as follows: beans (22.6%), Yam (44.4%), garri (50%) pears (83.3%), cassava “foo-foo” (16.7%), “abacha” (83.3%), “okpa” (56%), rice (56%), “agidi” (83.3%) and moi-moi (66.7%). Out of the ten water samples, six were also positive for Escherichia coli. Escherichia coli high proportion of food (75.9%) and water (80%) respectively showed the presence of coliforms in general including Escherichia coli Geometric mean count (GMC) for MPN ranged from 1-180 and GMC for coliforms in various foods ranged from 1.0 x 101-1.0 x 106cfu/g. About 42.4% human coliforms (Escherichia coli), 37.4% of faecal coliforms (Klebsiella pneumonia) and rest (20.2%) showed other coliforms in food and water samples.
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References
Abdullahi, I.O., Umoh, V.J, Ameh, J.B. and Galadima ,M. (2004). Hazards associated with kilishi preparation in Zaria, Nigeria. Nigerian Journal of Microbiology, 18(1/2): 339-345.
Adam, M.R and Moss, M.O. (1996). Methods for the Microbiological Examination of Foods. In: Food Microbiology, Royal Society of Chemistry, Cambridge, pp 303-322.
Adesiyun, A.A.(1984). Prevalence and characterization of Staphylococcus from five ready-to-eat products in Nigeria. Nigerian Food Journal, 2(2): 135-139.
Adesiyun, A.A. (1995). Bacteriological quality of some Trinidadian ready-to-consume foods and drinks and possible health risks to consumers. Journal of Food Protection, 58: 651-655.
Adesiyun, A.A. and Kwaya, J.K.P. (1983). Microbial quality and potential of Staphylococcal growth in some consumed constituted infant food. Nigerian Food Journal, 1(1): 1118-120.
Ahmed, Z.I., Poahmi, A. and Siddiqui, S.C. (1976). Bacteriology examination drinking water of Karachi and Isolation of enteric pathogens.
Pakistan Journal of Science Industrial Research, 7: 103-110.
Altekruse, S.F., Street, D.A., Flein, S.B. and Levy, (1995). Consumer knowledge of food borne microbial hazards and food handling practices. Journal of Food Protection, 5: 66-73.
APHA (1998). Analytical Methods for Total Coliforms. In: Standard Methods for the Examination of Water and Wastewater, American Public Health Association.
Black, RA.S..E., Merson, M.H., Rahman, A.S.M., Yunus, M., Alim, A.R., Uhg, M.I., Holken, I. and Culin, R.H. (1980). Viral and parasitic agents associated with diarrhoea in rural, Bangladeshi Journal Infectious Diseases, 142: 660-664.
Brooks, G.F., Butel, J.S. and Morse, S.A. (2004). Enteric Gram Negative Rods (Enterobacteriaceae). In: Jawetz, Melnick and Adelberg‟s Medical Microbiology. 23rd Int‟I Edition. McGraw Hill Education Companies Inc. Singapore, pp 248-261.
Byran, F.L.C. (1988). Risks of practices, procedures and processes that lead to outbreaks of food borne diseases. Journal of Food Protection, 59: 287-294.
Cheesbrough, M. (1993). Bacteriological Testing of Water Supplies: Probability Tables for Estimating with MPN of Faecal Coliform Bacteria. Medical Laboratory Manual for Tropical Countries. Educational Low Priced Books Scheme (ELBS). pp 219-220.
Clark, J.A. and Pagel, J.C. (1997). Pollution indicator bacteria associated with municipal raw water and drinking water supplies. Canadian Journal Microbiology, 23: 465-470.
Davidson, S., Passmore, R., Brook, J.F. and Truswell, A.S. (1975). Human Nutrition and Dietetics. 6th Ed., Churchill Livingstone, Edinburgh.
Dibua, U.E., Esimone, C.O. and Ndianefo, P.C. (2007). Microbiological and physiochemical characterization of satchet water samples marketed in Nsukka Campus of the University of Nigeria. Bio-Research, 5(1): 189-193.
Dosumu, O.O., Oluwanyi, O.O., Awolola, G.V. and Okunola, M.O. (2009). Stability studies and mineral concentrations of some Nigerian packed fruit juices, concentrate and local beverages. African Journal of Food Science, 3(3): 082-085.
Enwere, N.J. (1998). What is Food? In: Foods of Plant Origin, Afro-Orbis Publ. Ltd., Nigeria, pp 3-21.
Feng, P.C.S. and Hartman, P.A.C. (1982). Fluorogenic assays for immediate confirmation of Escherichia coli. Applied and Environmental Microbiology, 43(6): 1320-1329.
Frazier, W.C. and Westhoff, D.C. (2005). Food as a Substrate for Microorganisms. In: Food Microbiology. 4th Ed., Tata McGraw-Hill Publ. Co. Ltd., New Delhi. pp 3-16.
Harrigan, W.F. and McCance, M.E. (1976). Biochemical tests for Bacteria. In: Laboratory Methods in Food and Dairy Microbiology, Academic Press, London. pp 66-82.
Henry, W., Sealey, J. and Demark, P.J.V. (1987). Staining Techniques: Gram Stain. In: Selected Exercises from Microbes in Action, 2nd Ed. W.H. Freeman and Co., USA.
ICMSF (1978). International Commission on Microbiological Specification of Foods 2: Sampling for Microbiological Analysis Principles and Specific applications. University of Toronto Press. pp 119-118.
Jay, J.M. (2004). History of Microorganisms in Food. In: Modern Food Microbiology. 4th Ed. CBS Publ., New Dehli. pp 3-10.
Jiwa, S.F., Krovacek, K. and Wadstrim, T. (1981). Enterotoxigenic bacteria in food and water from an Ethiopian Community. Applied and Environmental Microbiology. 41(4): 3-16.
LeChavellier, M.W. and McFeters, G.A. (1984). Recent advances in coliform methodology for water analysis. Journal of Environmental Health, 47(1): 5-9.
Mahale, D.P., Khade, R.G. and Vaidya, V.K. (2008). Microbiological analysis of street vended fruit Juices from Mumbai City, India, Internet Journal of Food Safety, 10: 31-34.
Mermin, J.H., Villar, R., Carpenter, J., Robert, L., Samaridden, A., Gasanova, L., Lomakina, S., Bopp, C., Hutwagner, L., Mead, P., Ross, B. and Mintz, E.D. (1999). A massive epidermic of multi-drug-resistant typhoid fever in Tajikistan Association with consumption of municipal water. The
Journal of Infectious Diseases, 176: 1416-1422.
Okaka, J.C. and Ene, G.I. (2005). Microorganisms–Implications of Presence in Foods. In: Food Microbiology- Methods in Food Safety Control, Ocjanco Academic Publication, Nigeria. pp 1-12.
Oxoid (1982). The Oxoid Manual of Culture Media, Ingredients and Other laboratory Services, 5th Ed., Oxoid Limited, Basingstoke.
Prescott, L.M., Harley, J.P and Klein, D.E. (1996). The Study of Microbial, Structure, Microscopy and Specimen Preparation. In: Microbiology, 7th ed. McGraw-Hill, New York. pp 17-36.
Sadiq, M. and Abdullahi, I.O. (2008). Hygienic evaluation of two food service centres in a University campus in Samaru, Zaria, Nigeria. Nigerian Food Journal, 26(1): 71-76.
Speck, M.L. (Ed.) (1988). Compendium of Methods for the Microbiological Examination of Foods. American Public Health Association ,Washington D.C.. pp 200-500.
WHO (1971). International standard of drinking water. WHO Journal, pp 3.
WHO(1981). Global Strategy for Health for all by the year 2000. WHO, Geneva, pp7-13.
WHO(1993). Contaminated Food: A Major Cause of Diarrhoea associated with Malnutrition among Infants and Young Children. WHO Journal
Windle, T.E. (1968). The Examination of Water and Water Supplies. 42nd Report of the Metropolitan Water Boards, 7th Ed;
Churchill Publ., London, pp 117.
Zammelli, P.Z., Brabbia, C.A. and Gathia, G.E. (1993). Air pollution associated with food spoilage. Journal of Food Protection, 12: 105-109.
Zweitering,M.H.(2002). Quantification of Microbial quality and safety in minimally processed food. Dairy Journal, 12: 264-271.