Changes in the enzymes, amino acids, metal ions and flavor profile during fermentation of African locust bean seeds ‘Parkia biglobosa’ to Daddawa
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Abstract
Daddawa/iru, a product of African locust bean (Parkia biglobosa) seeds, is widely used as food condiment by many tribes in West Africa. This study aimed to determine some of the changes which occur during the microbial fermentation of African locust bean seed. The daily changes in pH, amylase, lipase and protease secretion were monitored. Changes in amino acid concentration, mineral ion composition were also monitored; the presence of hydrocarbons and volatile flavor compounds were also determined. The pH increased throughout fermentation and was above 8.5 by 96 h fermentation. Enzyme production was highest after 48 h fermentation. The highest increase in amino acid concentration was observed after 72 h fermentation. The essential amino acids which increased after 72 h fermentation include arginine (48.8%), threonine (25.2%), valine (24.8%), and methionine (20.0%), histidine (10.3%), isoleucine (19.2%), leucine
(15.9%), lysine (15.24%), phenylalanine (14.9%), and tryptophan (12.5%). A higher metal ion concentration was observed before fermentation (0 h) than during or after fermentation of the locust bean seeds. Various hydrocarbons, fatty acids, esters and alcohols were observed at different stages of the fermentation.
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References
Achi, O.K. (1992). Microorganisms associated with natural fermentation of Prosopis africana seeds for the production of okpiye. Plant Food Human Nutrition. 42:297–304.
Adebiyi, J. A., Njobeh, P. B., Adebo, O. A. and Kayitesi, E. (2021). Metabolite profile of Bambara groundnut (Vigna subterranea) and dawadawa (an
African fermented condiment) investigation using gas chromatography high resolution timeof-flight mass spectrometry (GCHRTOF-MS). Heliyon. 7(4): e06666.
Adelekan, A.O. and Nwadiuto, E. (2012). Bacterial succession studies during fermentation of African locust bean (Parkia biglobosa) to iru using
molecular methods. British Biotechnology Journal. 2(1): 49-59.
Akhbari, M., Batooli, H. and Kashi, F.J. (2012). Composition of essential oil and biological activity of extracts of Viola odorata L. from central Iranian. Natural Product Research. 26(9): 802-809.
Akinwumi, B.C., Bordun, K.M. and Anderson, H.D. (2018). Biological of stilbenoids. International Journal of Molecular Science. 19(3):792.
Amao, J.A., Odunfa, S.A. and Mbom, C.N. (2018). The role of Staphylococcus species in the production of iru during the fermentation of African locust beans (Parkia biglobosa). Food Research. 2(2): 187-193.
AOAC (2006). Official methods of analysis. 18th Edition, Association of Official Analytical Chemists, Gaithersburgs, MD.
Awodi, P. S., Nwagu, T. N., Tivkaa, J. A, Ella, B. and Ogbonna, J. C. (2021). Simultaneous saccharifcation and fermentation of pawpaw (Carica
papaya) seeds for ethanol production· Vegetos. doi.org/10.1007/s42535-021-00231-z
Azokpota, P., Hounhouigan, D.J. and Nago, M.C. (2006). Microbiological and chemical during the fermentation of African locust bean (Parkia biglobosa) to produce afitin, iru and sonru, three traditional condiments produced in Benin. International Jouranl of Food Microbiology. 107(3): 304-309.
Benitez, L.V. (1989). Amino Acid and fatty acid profiles in aquaculture nutrition studies. In: S.S. De Silva (ed.) Fish Nutrition Research in Asia: Proceedings of the Third Asian Fish Nutrition Network Meeting. Manila, Philippines, Asian Fisheries Society pp:23-35.
Bhunia, B., Basak, B. and Dey, A. (2012). A review on production ofserine alkaline protease by Bacillus spp. Journal of Biochemical Technology. 3(4): 448-457.
Caputi, L., Aprea, T. (2011). Use of terpenoids as natural flavouring compounds in food industry. Recent Patents in Food Nutrition and Agriculture,
Contesini, F.J., Melo, R.R. and Sato, H.H. (2018). An overview of Bacillus proteases: from production to application. Critical Reviews in Biotechnology. 38(3): 321-324.
Diomandé, S.E., Nguyen-The, C., Guinebretière, M.H., Broussolle, V. and Brillard, J. (2015). Role of fatty acids in Bacillus environmental adaptation. Frontiers in Microbiology. 6: 813.
Edema, M.O., Fawole, O. (2006). Evaluation and optimization of critical control points in the production of iru. Research Journal in Microbiolog . 1: 503-511.
Ezeokoli, O.T., Adeleke, R.A. and Bezuidenhout, C.C. (2018). Core bacterial community of soy-daddawa: Insights from high-throughput DNA
metabarcoding. LWT. 97: 61-66.
Farinde, E.O., Abiose, S.H. and Adeniran, H.A. (2021). Diversity of bacteria during fermentation of limabean into daddawa. Journal of Microbiology, Biotechnology and Food Sciences. 2021:1228-1232.
Fapojuwo, O.O., Afolabi, O.A., Iheanacho, K.E. and Maga, J.A. (1986). Nature of lipids in African locust beans (Parkia filicoidea Welw.) and changes
occurring during processing and storage. Journal of Agriculture and Food Chemistry. 34: 246–248.
Hayek, S.A. and Ibrahim, S.A. (2013). Current limitations and challenges with lactic acid bacteria: A review. Food and Nutriton Science. 4: 73-87.
Hou, C.T. (2009). Biotechnology for fats and oils: new oxygenated fatty acids. New Biotechnology. 26: 2-10.
Ijarotimi, O.S. and Keshinro, O.O. (2012). Comparison between the amino acid, fatty acid mineral and nutritional quality of raw, germinated and fermented African locust bean (Parkia biglobosa) flour. Acta Scientiarum Polonorum Technologia Alimentarum. 11(2): 151-165.
Isichei, M.O. and Achinewhu, S.C. (1988). The nutritive value of African oil bean seeds (Pentaclethra macrophylla). Food Chemistry. 30(22): 83-92.
Jelen, H., Majcher, M., Ginja, A. And Kuligowski, M. (2013). Determination of compounds responsible for tempeh aroma. Food Chemistry. 141(1): 459-465.
Kolodziejski, D., Koss-Mikokajck, I., Abdin, A.Y., Jacob, C. and Bartoszek, A. (2019). Chemical Aspects of Biological Activity of Isothiocyanates and Indoles, the Products of Glucosinolate Decomposition.Current Pharmaceutical Design. 25(15): 1717-1728.
Ndamitso, M.M., Musah, M., Mathew, J.T. and Bissala, V.T. (2020). Comparative Nutritional Analysis of Daddawa Made from Fermented Parkia biglobosa and Glycine max Seeds. Comminication in Physical Science. 5: 263-269.
Nwagu, T. and Amadi, O.C. (2013). Amylolytic, lipolytic and proteolytic activity of Kocuria varians isolated from fermented African oil bean seed
(Pentaclethra macrophylla). Scientific Journal of Microbiology. 2: 65-73.
Nwagu, T.N., Amadi, E.C. and Alaekwe, O. (2010). Role of bacterial isolates in the spoilage of fermented African oil bean seed ‘ugba’. Pakistan Journal of Biological Science. 13: 497-503.
Nwagu, T.N., Orji, M.U., Nwobodo, I. And Nwobodo, H.A. (2011). Mixed microbial flora as starter culture for the production of ‘ugba’ from African oil bean seed. Asian Journal of Biological Science. 4: 62-69.
Nwagu, T.N., Ugwuodo, C.J., Onwosi, C.O., Inyima, O., Uchendu, O.C. and Akpuru, C. (2020). Evaluation of the probiotic attributes of Bacillus strains isolated from traditional fermented African locust bean seeds (Parkia biglobosa),“daddawa”. Annals of Microbiology. 70(1): 1-15.
Nwokeleme, C.O. and Ugwuanyi, J.O. (2014). Evolution of volatile flavour compounds during fermentation of African Oil Bean (Pentaclethra macrophylla Benth) seeds for “ugba” production. International Journal of Food Science.
Odibo, F.J.C., Ezeaku, E.O. and Ogbo, F.C. (2008). Biochemical changes during the fermentation of Prosopis africanaseeds for ogiri-okpei production. Journal of Industrial Microbiology and Biotechnology. 35(9): 947-952.
Odunfa, S.A. (1985). Biochemical changes during ‘iru’ fermentation. International Journal of Food Technology. 20: 295-303.
Ohenhen, R.I., Imarenezor, E.P., Iyamu,M.I. and Aigbokhan, F.I. (2008). A comparison of preservation methods of traditionally processed dawadawa. Continental Journal of Microbiology. 2: 11-15.
Oluwaniyi, O.O. and Bazambo, I.O. (2016). Nutritional and amino acid analysis of raw, partially fermented and completely fermented locust bean
(Parkia biglobosa seeds). African Journal of Food, Agriculture and Nutritional Development. 16(2): 10867-10883.
Omafuvbe, B.O., Abiose, S.H. and Shounkan, O.O. (2002). Fermentation of soybean (Glycine max) for soy daddawa production by starter cultures of Bacillus. Food Microbiology. 9: 561-566.
Omafuvbe, B.O., Falade, O.S., Osuntogun, B.A. and Adewusi, S.R.A. (2004).Chemical and Biochemical Changes in African Locust Beans (Parkia biglobosa) and Melon (Citrullus vulgaris) Seed sduring Fermentation to Condiments. Pakistan Journal of Nutrition. 3(3): 140-145.
Robel, E.J. (1967). Ion-Exchange chromatograph for the determination of tryptophan. Analytical Biochemistry. 18(3): 406-413.
Sarkar, P.K., Hasenack, B. and Nout, M.J. (2002). Diversity and functionality of Bacillus and related genera isolated from spontaneously fermented
soybeans (Indian Kinema) and locust beans (African Soumbala). International Journal of Food Microbiology. 77: 175-186.
Sharon-Asa, L., Shalit, M., Frydman, A., Bar, E., Holland, D., Or, E., Lavi, U., Lewinsohn, E. and Eyal, Y. (2003). Citrus fruit flavour and aroma
biosynthesis: isolation, functional characterization, and developmental regulation of Cstps1, a key gene in the production of sesquiterpene aroma compound valence. Plant Journal. 36(5): 664-674.
Verardo, V., Gomez-Caravaca, A.M., Tabanelli, G. (2020). Bioactive components in fermented foods and food by-products. Foods. 9: 153.
Yakubu, C. M., Rajan, S. and Savita S. (2022a). Fermentation of locust bean (Parkia biglobosa): modulation in the anti‐nutrient composition, bioactive profile, in vitro nutrient digestibility, functional and morphological characteristics. International Journal of Food Science & Technology. 57: 753-762.
Yakubu, C.M., Sharma, R., Sharma, S. and Singh, B. (2022b). Influence of alkaline fermentation time on in vitro nutrient digestibility, bio-& techno-functionality, secondary protein structure and macromolecular morphology of locust bean (Parkia biglobosa) flour. LWT. 113295.
Yves, A.K., Honore, O.G., Germain, K.T. and Zeze, G.K. (2019). Molecular identification and species diversity of the microbiota associated with
soumbara, a traditional fermented food commonly consumed in Cote d’Ivoire. Research Journal in Food Science and Nutrition. 4: 48-57.
Zviely, M. and Hong, C. (2009). Hydrocarbons: significance in the flavour and fragrance industries. https://www.researchgate.net/publicati
on/235430229_Hydrocarbons_signifiance_in the flavor and fragrance industries. Retrieved on the 17th of July 2019.