Improvement of Provitamin A in Maize Varieties Using Arbuscular Mycorrhizal Fungus, Glomus clarum
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Abstract
Arbuscular mycorrhizal fungus (AMF, Glomus clarum) has been used widely as a bio-amendment and bio-control agent in several biotechnological studies. In this study, biofortification of maize with provitamin A using AMF was investigated. Five maize varieties (V1 = white drought-resistant maize, V2= yellow provitamin A maize, V3= white drought-tolerant maize, V4= yellow striga-resistant maize and V5= white striga-resistant maize) were evaluated in a screen house experiment laid out in a completely randomized design with three treatments: T1 = maize + AMF before planting, T2 = maize + AMF, inoculated two weeks after planting and T3 (control) = maize only, and four replications. The result showed that AMF significantly (p<0.05, p = 0.0029) increased the provitamin A level of the maize varieties. White drought-tolerant maize (V3) had the highest provitamin A content (581.57 µg) after harvest, while the least (288.33 µg) was found in white drought-resistant maize (V1). Also, the effect of the treatments on the growth traits (plant height, leaf length, number of leaves per plant) of the maize varieties was highly significant. Therefore, AMF could be considered in breeding maize with high provitamin A content and improved morphological characters.
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References
Abiala, M. A, Popoola, O. O., Olawuyi, O. J., Oyelude, O. J., Akanmu, A. O., Killani, A. S., Osonubi, O. and Odebode, A.
C. (2013). Harnessing the potentials of vesicular arbuscular mycorrhizal (VAM) fungi to plant growth – a review. International Journal of Pure Inoculated Science and Technology 14:61–79.
Ahanger, M. A., Tyagi, S. R., Wani, M. R. and Ahmad, P. (2014). Drought tolerance: role of organic osmolytes, growth regulators and mineral nutrients in physiological mechanisms and adaptation strategies in plants under changing environment, vol. 1. Eds. Ahmad, P., Wani, M. R (New York, NY: Springer), Pp. 25-55.
Aremu, S. O. and Nweze, C. C. (2017). Determination of vitamin A content fromNigerian fruits using spectrophotometric
method. Bangladesh Journal of Scientific and Industrial Research 52(2): 153-158.
Birhane, E., Sterck, F., Fetene, M., Bongers, F. and Kuyper, T. W. (2012). Arbuscular mycorrhizal fungi enhance photosynthesis, water use efficiency, and growth of Frankincense seedlings under pulsed water availability conditions. Oecologia 169 (4): 895- 904.
Bouis, H. E. and Saltzman, A. (2017). Improving nutrition through biofortification: A review of evidence from HarvestPlus, 2003 through 2016. Global Food Security, 12: 49-58.
Chen, M., Arato, M., Borghi, L., Nouri, E. and Reinhardt, D. (2018). Beneficial services of arbuscular mycorrhizal fungi- from ecology to application. Frontiers in Plant Science, 9: 1270.
Cuttriss, A. J., Cazzonelli, C. I., Wurtzel, E. T. and Pogson, B. J. (2011). Biosynthesis in Plants Part A. Advances in Botanical
Research, 58; 1-36.
Giuliano, G. (2017). Provitamin A biofortification of crop plants: a gold rush with many miners. Current Opinion in
Biotechnology 44: 169-180.
Harrier, L. (2001). The arbuscular mycorrhizal symbiosis: a molecular review of the fungal dimension. Journal of
Experimental Botany 52: 469-478.
Hart, M., Ehret, D. L., Krumbein, A., Leung, C., Murch, S., Turi, C. et al. (2015). Inoculation with arbuscular mycorrhizal fungi improves the nutritional value of tomatoes. Mycorrhiza 25: 359-376.
HarvestPlus (2014). Kigali declaration on biofortified nutritious food. Second Global Conference on Biofortification March 31st - April 2nd, 2014. Kigali Rwanda.
Heuze, V., Tran, G., Edouard, N., Lebas, F. (2017). Maize green forage. Bio-Research Vol.19 No.1 pp.1227-1236 (2021)
Feedipedia, a programme by INRA, CIRAD, AFZ and FAO. http://www.feedipedia.org/node/358. retrieved on February 19, 2021.
Jeffries, P., Gianinazzi, S., Perotto, S., Turnau, K. and Barea, J.M. (2003). The contribution of arbuscular mycorrhizal
fungi in sustainable maintenance of plant health and soil fertility. Biology and Fertility of Soils, 37: 1-16.
Kuhnen, S., Lemos, P.M., Campestrini, L. H., Ogliari, J. B., Dias, P. F. and Maraschin, M. (2011). Carotenoid and anthocyanin contents of grains of Brazilian maize landraces. Journal of Science, Food and Agriculture, 91(9): 1548-53.
Li, H., Smith, S. E., Holloway, R. E., Zhu, Y. and Smith, F. A. (2006). Arbuscular mycorrhizal fungi contribute to phosphorus uptake by wheat grown in a phosphorus-fixing soil even in the absence of positive growth responses. New Phytologist 172: 536- 543.
Liggett, R. W. and Koffler, H. (1948). Corn steep liquor in microbiology. Bacteriology Reviews 12: 297-311.
Liu, A., Hamel, C., Begna, S. H., Ma, B. L. and Smith, D. L. (2000). Mycorrhizae formation and nutrient uptake of new corn (Zea mays L.) hybrids with extreme canopy and leaf architecture as influenced by soil N and P levels. Plant and Soil 221: 157-166.
Nakmee, P. S., Techapinyawat, S. and Ngamprasit, S. (2016) Comparative potentials of native arbuscular mycorrhizal fungi to improve nutrient uptake and biomass of sorghum bicolor Linn. Agriculture and Natural Resources, 50: 173-178.
Oganization for Economic Co-operation and Development (2006).Safety Assessment of Transgenic Organisms. OECD Consensus Documents 1: 52pp Olakojo, S. A., Omueti, O., Ajomale, K. and Ogunbodede, B. A. (2007).
Development of quality maize: biochemical and agronomic evaluation. Tropical and Sub-tropical Agroecosystems 7 (2): 97-104.
Olawuyi, O. J. and Onuoah, S. O. (2017). Genetic assessment of Amaranthus Linn. Genotypes in treatment combinations of Glomus clarum and Leucaena leucocephala Lam. Using Simple Sequence Repeat (SSR) Marker. Molecular Plant Breeding 8 (10): 53- 76.
Olawuyi, O. J., Odebode, A. C., Oyewole, I. O., Akanmu, A. O. and Afolabi, O. (2013). Effect of Arbuscular Mycorrhizal Fungi
on Pythium aphaerosidamium causing foot rot in Pawpaw (Carica papaya I.) seedlings. Archives of Phytopathology and Plant Protection, 40: 185-193.
Olawuyi, O. J., Bello, O. B., Ntube, C. V. and Akanmu, A. O. (2015). Progress from selection of some maize cultivars’
response to drought in the dried Savanna of Nigeria. Agrivita 37(1): 8- 17.
Olawuyi, O. J., Ezekiel-Adewoyin, D. T., Odebode, A. C., Aina, D. A. and Esenbamen, G. (2012). Effects of arbuscular
mycorrhiza (Glomus clarum) and organomineral fertilizer on growth and yield performance of okra (Abelmoschus esculentus). African Journal of Plant Sciences, 6 (2): 84-88.
Olawuyi, O. J., Jonathan, S. G., Babatunde, F. E., Babalola, B. J., Yaya, O. S., Agbolade, J. O., Aina, D. A. and Egun, C. J. (2014). Accession, treatment interaction, variability and correlation studies of pepper (Capsicum spp.) under the influence of arbuscular mycorrrhizal fungus (Glomus clarum) and cow dung. American Journal of Plant Sciences 5: 683-690.
Olawuyi, O. J., Odebode, A. C., Alfar, A., Olakojo, S. A. and Adesoye, A. I. (2010). Performance of maize genotypes and arbuscular mycorrhizal fungi in Samara District of Southwest Region of Dohar-Qatar. Nigerian Journal of Mycology, 3: 86-100.
Olawuyi, O. J., Odebode, A. C., Olakojo, S. A. and Adesoye, A. I. (2011). Host- parasite relationship of maize (Zea mays L.) and Striga lutea (lour) as influenced by arbuscular mycorrhiza fungi. Journal of Science, 10(2): 186- 198.
Olawuyi, O. J., Odebode, A.C., Olakojo, S.A., Popoola, O. O. and Akanmu, A. O. and Izenigu, J. O. (2014). Host pathogen interaction of maize (Zea Bio-Research Vol.19 No.1 pp.1227-1236 (2021) mays L.) and Aspergillus niger as influenced by mycorrhizal fungi (Glomus deserticola) Archives of Agronomy and Soil Science. 60 (11): 1577-1591.
Olowe, O. M., Asemoloye, M. D. and Olawuyi, O. J. (2020). Newly identified Fusarium strains (olowlLH1 and olowlLH2) causing ear rot maize and their control using Glomus clarumand G. deserticola. Plant Biosystems. Doi: 10.1080/11263504.2020.1762780.
Ortas, I. (2010). Effects of mycorrhizal application on plant growth and nutrient uptake im cucumber production under field conditions. Spanish Journal of Agricultural Research 8 (S1): S116- S122.
Rodrigues, K. M. and Rodrigues, B. F (2014). Arbuscular mycorrhizal (AM) fungi and plant health. Fungi in Biotechnology, M. Gosavi (ed.).
SIES College, Sion, Mumbai; 8-24.8-24. Rouphael, Y., Franken, P., Schneider, C., Schwarz, D., Giovannetti, M. and
Agnolucci, M. (2015). Arbuscular mycorrhizal fungi act as bio- stimulants in horticultural crops. Scientia Horticulturae, 96: 91-108
Salam, E. A., Alatar, A., El-Sheikh, M. A. (2017). Inoculation with arbuscular mycorrhizal fungi alleviates harmful effects of drought stress on damask rose. Saudi Journal of Biological Sciences 25 (8): 1772-1780.
Salam, L. B. and Ishaq, A. (2019). Biostimulation potentials of corn steep liquor in enhanced hydrocarbon degradation in chronically polluted soil. Biotechnology, 9 (2): 46-50.
Sbrana, C., Avio, L. and Giovannetti, M. (2014). Beneficial mycorrhizal symbionts affecting the production of health-promoting phytochemicals. Electrophoresis 35: 1535-1546.
Scott, M. P. and Emery, M. (2016). Reference Module in Food Science. Smith, S.E. and Read, D.J. (1997) Mycorrhizal Symbiosis. 2nd Edition. Academic press, London, UK., ISBN-13:978-0- 12-652840-4, Pages: 605.
Smith, S.E. and Read, D.J. (2008) Mycorrhizal Symbiosis. 3rd Edition. Academic press, London, UK. ISBN- 13:9780123705266, Pages: 800. Elsevier. ISBN: 9780081005965. https://doi.org/10.1016/B978-0-08- 100596-5.00022-6.
Trouvelot, S., Bonneau, L., Redecker, D., Van Tuinen, D., Adrian, M. and Wipf, D. (2015). Arbuscular mycorrhizal
symbiosis in viticulture: A review. Agronomy for Sustainable Development,. 35: 1449-1467.
Bio-Research Vol.19 No.1 pp.1227-1236 (2021) Uchendu, F. N. (2013). The role of biofortification in the reduction of micronutrient food insecurity in developing countries. African Journal of Biotechnology 12 (37): 5559-5566
White, P. J. and Broadley, M. R. (2005). Bio- fortifying crops with essential mineral element. Trends in Plant Science 10: 586-593.