Nutritional changes induced by fungi on cowpea (Vigna unguiculata L. Walp) seeds
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Abstract
Seeds are usually infected by microorganisms and pests during storage, causing deterioration and reduction in the nutritive and market value of these seeds. In this study, the proximate composition of Vigna unguiculata seeds inoculated with different fungal organisms was determined to ascertain the level of deterioration caused by fungi on the seeds. The fungi used in the study were Botryodiplodia theobromae, Fusarium oxysporum, Rhizopus stolonifer and Aspergillus niger. There was a significant increase (p<0.05, 0.008) in the protein content of seeds inoculated with fungi. Fusarium oxysporum (29.45%) caused the highest increase in protein followed by Aspergillus niger (28.14%), Botryodiplodia theobromae (27.85%) and Rhizopus stolonifer (27.50%). The increase could be attributed to the proteineous content of the fungal mycelia. There was a significant increase (p<0.05, 0.005/0.014) in moisture and ash content of inoculated seeds respectively. Fusarium oxysporum caused the highest increase in ash (7.93) while Rhizopus stolonifer (5.4) caused the
lowest increase. The increase in ash content is due to the presence of minerals like potassium and phosphorus in the mycelia of the fungi. There was a significant decrease (p<0.05, 0.019) in the carbohydrate, lipid, fibre and dry matter content of fungi-inoculated seeds when compared with the control. Fusarium oxysporum (36.6) caused the highest decrease while Rhizopus stolonifer (43.2) caused the lowest decrease in dry matter of inoculated seeds. Decrease in dry matter may be as a result of production of enzymes by these fungi.
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References
Addo-quate, A. A., Darkwa, A. A. and Ampiah, M. K. P. (2011). Performance of three cowpea (Vigna unguiculata (L.) Walp) varieties in
two agroecological zones of the central region of Ghanna I: dry matter production and growth analysis. Journal of Agric and Biological Sciences, 6(2): 1990 – 1995.
Adebayo, R. A., Adegbenro, T. A. and Hassan, G. F. (2020). Occurrence of pathogens on grains of cowpea (Vigna unguiculata Walpers) and maize (Zea mays L.) infested by Callosobruchus maculatuss (F.) and Sitophilus zeamais Mots. Acta Scientific Agriculture, 4(1): 1-8
Afolabi, C. G., Ezekiel, C. N., Ogunbiyi, A. E., Oluwadairo, O. J., Sulyok, M. and Krska, R. (2020). Fungi and mycotoxins in cowpea (Vigna Unguiculata) on Nigerian markets. Food Audit Contaminations Part B. Surveillance, 13(1): 52-58
Al-Jaradi, A., Al-Mahmooli, I., Janke, R., Maharachchikumbura, S., Al-Saady, N., AlSadi, A. M. (2018). Isolation and Identification of pathogenic fungi and oomycetes associated with beans and cowpea root diseases in Oman. PeerJ Journal of Life and Environmental Sciences, 6: 1-21
AOAC (1995). Official methods of analysis (16th edition). Association of Analytical Chemists. Virginia, USA 1018p.
Ataga, A. E., Elenwo, E. N. and Nwachukwu, E. O. (2010). Laboratory exercises and series in mycology. ACOTEC Technologies, Port Harcourt, Nigeria, 58 – 64 pp. Ataga, A. E. and Umechuruba, C. I. (1997).
Biochemical changes in African yam bean seeds caused by Botryodiplodia theobromae, Fusarium palidoresum and Penicillium exaliam. Global Journal of Pure and Applied Sciences, 4(4): 381 – 384.
Aziz, N. H. and Mahrous, S. R. (2004). Effect of gamma irradiation aflatoxin B1 production by Aspergillus flavus and chemical composition of three crop seeds. Nahrunig Wiely Velt Verlag GMBtt & Co. Kga A, Weinheim, Germany, 48: 234-238.
Boukar, O., Belko, N., Chamarthi, S., Togola, A., Batieno, J., Owusu, E., Haruna, M., Diallo, S., Umar, M., Olufajo, O. and Fatok8un, C. (2017). Cowpea (Vigna unguiculata): genetics, genomics and breeding. Plant Breeding, 1-10.
Directorate Plant Production (2011). Production Guidelines for Cowpea. Directorate Agricultural Information Services Pretoria, South Africa. 16pp.
Elhardallou, S. B., Khalid, I. B., Gobouri, A. A. and Abdel-Hafez, S. H. (2015). Amino acid composition of cowpea (Vigna ungiculata L. Walp) flour and its protein isolates. Food and Nutrition Sciences, 6(9): 790-797.
Embaby, E. M., Mohamed, R., mosaad, A. A., Hassan, O. And Asman, M. M. (2013). Occurrence of toxigenic fungi and mycotoxins in some legume seeds. Journal of Agricultural Technology, 9(1): 151-164.
Enwere, J. N. (1998). Plant Origin. Afro-Orbis Publisher, Nsukka, pp: 24, 25, 240. FAOStat (2017). FAOSTAT, Statistical data base. Rome: Food and Agricultural Organizations of the United Nations. Visited on 15/08/2017.
Gogile, A., Andargie, M. and Muthuswamy, M. (2013). Screening selected genotypes of cowpea [Vigna unguiculata (L.) Walp.] for salt tolerance during seedling growth stage. Pakistan Journal of Biological Sciences,16 (14): 671-679.
Hall, A. E. (2012). Phenotyping cowpeas for adaptation to drought. Frontiers in Physiology, 3:1-8.
ISTA (International Seed Testing Association) (2016). International rules for seed testing - rules ammendments. Seed Science Technology, 29: 1-127.
Jonbozorgi, S., Mehrabi-Koushki, M. and Farokhinejad, R. (2019). Isolation and identification of fungal endophytes of the cowpea in Khuzestan province. Biological Journal of Microorganisms, 8(28): 1-21
Kakde, R. B. and Chavan, A. M. (2011). Deteriorative changes in oil seeds due to storage fungi and efficacy of botanicals. Current Botany, 2(1):17-22.
Khairnar, D. N. (2015). Studies on seed-borne fungi, bio-deterioration of seeds and control. International Research Journal of Science and Engineering, 3(2): 60-62.
Klement, Z. K. and Voros, I. C. (1974). Methods in Pathology. Elsevier Scientific Publishing Company, Amsterdam, London. Pp. 220- 228.
Marques, M. R., Fontanari, G. G., Pimenta, D. C., Soares-Freitas, R. M. and Arêas, J. A. (2015). Proteolytic hydrolysis of cowpea proteins is able to release peptides with hypocholesterolemic activity. Food Research International, 77(1): 43-48.
Martins, L. M., Xavier, G. R., Rangel, F. W., Ribeiro, J. R., Neves, M. C., Morgado, L. B. and Rumjanek, N.G. (2003). Contribution of biological fixation to cowpea: A strategy for improving seed yield in the semi-arid region of Brazil. Biology and Fertility of Soils, 38: 333 – 339.
Monday, N. A. G., and Ataga, A. E. (2005). The Role of Aspergillus niger, Aspergillus flavus and Fusarium oxysporum on the nutritional composition and contents of Cyperus esculentus L. (B.Sc. Thesis), Department of Plant Science and Biotechnology, University of Port Harcourt, Nigeria.
Nwaukwu I. A. and Ataga, A. E (2013). Biochemical changes induced by five pathogenic fungi on seeds of Hibiscus sabdariffa (yakwa).Scientia Africana, 11(2): 1-6
Nwaukwu, I. A. and Ikechi-Nwogu, C. (2012). Biochemical changes induced by the effect of six pathogenic fungi on Dialum guineense: black velvet edible fruit. Journal of Pharmacy and Biological Sciences, 2(4): 20 – 24.
Okonkwo, S. N., Amund, O. O. and Ogunsanya, C. O (1990). Microbial rotting and
preservation of banana fruit (Musa sapientim L.) in Nigeria. Microbes Letters,
: 175 – 176.
Onifade, A. K., Atum, H. N. and Adebolu, T. T. (2004). Nutrient enrichment of sweet potato (Ipomea batatas L.). Global Journal of Pure and Applied Sciences, 10(1): 31 – 36.
Orkunas, I., Marzak, J. and Stabiecki, M. (2005). Sucrose-induced lupine defence against Fusarium oxysporum: sucrose stimulated accumulation of iso-flavonoids as a defence response of lupine to Fusarium oxysporum. Plant Pathology and
Biochemistry, 43(4): 363-373.
Saxena, N., Rani, S. K. and Deepika, M. (2015). Biodeterioration of soybean (Glycine max L.) seeds during storage by fungi. International Journal of Current
Microbiology and Applied Sciences, 4(6): 1118-1126.
Srivastava, S., Srivastava, M., Kumar, R., Singh, B., Gupta, G. and Sinha, A. (2013).
Influence of seed mycoflora on lipid content of Jatropha curcas seeds during storage. National Journal of Life Science, 10(1): 05- 08.
Tripathi Y. C. and Kumar, H. (2020). Biochemical and physicochemical changes in seeds and fatty oil of Salvadora oleoides and Salvadora persica by soil borne fungi. Journal of Chemical Science and Chemical Engineering, 1(2):1-6.
Tshovhote, N. J., Nesamvuni, A. E., Raphulu, T. and Gous, R. M. (2003). The chemical composition, energy and amino acid digestibility of cowpeas used in poultry nutrition. South African Journal of Animal Sciences, 33(1): 65 – 69.
Umechuruba, C. I. and Elenwo, E. N. (1997). Diagnostic Techniques in Mycology with Practical Exercise. Belk Publishers, 30 – 42pp.