Assessment of bacterial isolates associated with mobile phones of meat sellers in selected markets in Benin city, Edo State, Nigeria
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Abstract
Mobile phones are essential components used to enhance social life and aid different professions. Swab samples were obtained from ninety-six (96) meat sellers' mobile phones in four (4) major markets; Edaiken, New Benin, Oba
and Ogida in Benin City, over a period of four months. Mobile phones were sampled in the morning (8am-10am) and evening (4pm-6pm) between September, 2018 and December, 2018. Samples were immediately transported to the laboratory for microbiological processing and analysis using standard methods. The isolates were enumerated and identified, and antibiotics susceptibility test was carried out before and after plasmid curing. The study revealed that the mean total heterotrophic bacterial counts ranged from 1.07 ± 0.22 x 104 CFU/mL in New Benin market to 5.60 ±
0.12 x 104 CFU/mL in Edaiken market. The mean total coliform counts ranged from 0.60 ± 0.15 x 104 CFU/mL in Ogida Market to 4.63 ± 0.61 x 104 CFU/mL in Oba Market. The total staphylococcal counts ranged from 0.27 ± 0.09 x 104 CFU/mL in Oba market to 3.00 ± 0 ± 1.30 x 104 CFU/mL in New Benin market. Staphylococcus epidermidis , Bacillus spp, Staphylococcus aureus, Klebsiella sp., Pseudomonas aeruginosa, Escherichia coli and Enterococcus sp. were recovered. All the bacterial isolates had multiple antibiotic resistance index greater than the minimum limit of 0.2, indicating that the isolates are of significant public health concern. Escherichia coli, Klebsiella spp, Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus spp all had multiple plasmids, according to the plasmid profile study. The existence of bacterial isolates linked to human diseases on the phones of meat vendors highlight their potential as fomites, which could lead to disease outbreaks resulting in infections with serious public health implications.
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References
Adesokan, H. and Raji, A. (2014). Safe MeatHandling Knowledge, Attitudes and Practices of Private and Government Meat Processing Plants' Workers: Implications for Future Policy. Journal of Preventive Medicine and Hygiene55(1):10-6.
Adeyemo, S.M. and Onilude, A.A. (2015). Plasmid curing and its effect on the growth and physiological characteristics of Lactobacillus plantarum isolated from fermented cereals. Journal of Microbiological Research 5(1): 11-22.
Akinyemi, K., Atapu, A., Adetona, O. and Coker, A. (2009). The potential role of mobile phones in the spread of bacterial infections. Journal of Infection in Developing Countries 3(8):628-632.
Al-Abdalall, A. H. (2010). Isolation and identification of microbes associated with mobile phones in Dammam in Eastern Saudi Arabia. Journal of Family and Community Medicine 17:11-14.
Amala, S. E. and Ade, A. J. (2015). Bacteria associated with toilets and office lock handles. International Journal of Epidemiology and Infection 3 (1):12-15.
Anyadoh-Nwadike, S. O., Eri, O., Nwaokoro, J. C. and Nwadike, P.O. (2011). Prevalence of Staphylococcus aureus within the hospital environment. Asian Journal of Medical and Pharmaceutical Research 1(1):17-21.
Bell, R.G. (1997). Distribution and sources of microbial contamination on beef carcasses. Journal of Applied Microbiology 88: 292-300
Bhoonderowa, A., Gookol, S. and Birjanjiz -Hurdoyal S. (2014). The importance of mobile phones in the possible transmission of bacterial infections in the community. Journal of Community Health 39:965-967.
Butcher, W. and Ulaeto D. (2005). Contact inactivation of bacteria by household disinfectants. Journal of Applied Microbiology 99(2): 279-284.
Cheesbrough, M. (2000). District Laboratory Practice in Tropical countries Part 2, 2nd edition, Cambridge University Press, United Kingdom, 64pp.
Clinical Laboratory Standard Institute (CLSI) (2017). Performance Standards for Antimicrobial Susceptibility Testing, 27th edition. CLSI supplement M100S. Wayne, Pennsylvania, 250pp.
Doron, S. and Davidson, L. (2011). Antimicrobial stewardship. Mayo Clinic Proceedings. 86:1113–1123.
Ekhaise, F. O., Ighosewe, O. U. and Ajakpovi, D. O (2008). Hospital indoor airborne microflora in private and government owned hospitals in Benin City, Nigeria. World Journal of Medical Sciences 3(1):19-23.
Ekrakene, T. and Igeleke, L.C. (2007). Microorganisms associated with public mobile phones along Benin-Sapele Express Way, Benin City, Edo State of Nigeria. Journal of Applied Sciences Research 23:354-385.
Elkholy, M. and Ewees, I. (2010). Mobile (Cellular) Phone contamination with nosocomial pathogens in intensive care units. The Medical Journal of Cairo University 2:1-5.
Famurewa, O. and David, O. M. (2009). Cell phones: a medium of transmission of bacterial pathogens. World Rural Observations 1(2): 69-72.
Hassan, A. N., Birt D. M. and Frank, J. F. (2004). Behavior of Listeria monocytogenes in a Pseudomonas putida biofilm on a condensate-forming surface. Journal of Food Protection 67(2): 322-327.
Ibrahim, I. I., Subri, K.A., Mohammed K. K. and Mohamood, S.K (2013). Antecedents stirring purchase intention of smartphone among adolescents in Perlis. International Journal of Academic Research in Business and Social Science 3 (13):84-97.
Idemudia, I.B. and Ekhaise, I.B. (2019). Bacteriological enumeration and air quality of three major Hospitals in Benin City, Nigeria. Journal of Science and Technology Research 1(2):77-86.
Iheagwara, M. C. and Okonkwo T. M. (2016). Influence of storage duration on stability and sensorial quality of dried beef product (kilishi). Journal of Food Processing Technology 7: 574-581.
Ilusanya, O., Adesanya, O., Adesemowo, A. and Amushan N. (2012). Personal hygiene and microbial contamination of mobile phones of food vendors in Ago-Iwoye Town, Ogun State, Nigeria. Pakistan Journal of Nutrition 11(3): 276-278.
Julien, C. K., Gabaze, G. A., Kouame, K., Stephanie, K. K., Bernard, Y.O. and Mireille, D. (2014). The antibiotic resistance profile of Salmonella spp.
isolated from the sewage of the campus of the University of Cocody, Abidjan, Cote d 'Ivoire. International Journal of Tropical Disease and Health 4(5):608-620.
Karabay, O., Kocoglu, E. and Tahtaci, M. (2007). The Role of Mobile Phones in the Spread of Bacteria Associated with Nosocomial Infections. Journal of Infection in Developing Countries 1:72-73.
Khan, S. and Shaikh A. (2012) Mobile phones: reservoir of infectious diseases in university premises. NED University Journal of Research 9(1): 35-43.
Kilic, I., Ozaslan, M., Karagoz, I., Zer, Y. and Davutoglu V. (2009). The microbial colonisation of mobile phone used by healthcare staff. Pakistan Journal of Biological Sciences 12(11): 882-884.
Kozaèinski, L., Hadiosmanoviæ, M. and Zdolec, N. (2006). Microbiological quality of poultry meat on the croatian market. Veterinarski Arhiv 76: 305–313.
Krumpernam, P. H. (1983). Multiple antibiotic resistance indexing Escherichia coli to identify risk sources of faecal contamination of foods. Applied Environmental Microbiology 46:165-170.
Kumar, P. and Aswathy, M. (2014). Identification of mobile phone associated pathogens. Kerala Journal of Orthopaedics 27(1):69-72.
Kusumrungum, H. D., Riboldi, G., Hazelogen, W.C. and Bumper, R. R. (2003). Survival of food borne pathogens on stainless steel surfaces and cross contamination to foods. International Journal of Food Microbiology 55:227-236.
Leung, M. H., Wilkins, D and Lee, P. K. (2015). Insights into the panmicrobiome: skin microbial communities of chinese individuals differ from other racial groups. Scientific Reports 5:11845.
Lubwama, M., Kateete, D.P., Avazika, K.T., Nalwanga,W., Kagambo, D.B., Nsubuga, M.D., Arach, A.K., Ssetaba, L.J., Wamala, J.N., Rwot. L.A. and
Kajumbula, H. (2021). Microbiological contamination of mobile phones and mobile phonehygien of final-year medical students in Uganda: a need for educational intervention. Advances in Medical Education and Practice 12: 1247-1257.
Mackowiak, K. P. (1982). The normal microbial flora. The New England Journal of Medicine 307(2):83-93.
Neubauer, G., Rooslim, K. and Wiart J. (2005). Study on feasibility of epidemiological studies on healt effects mobile telephone stations. Journal of Health Population and Nutrition 20(4): 200-210.
Osundiya, O., Oladele, R. and Oduyebo, O. (2013). Multiple antibiotic resistance (MAR Index of Pseudomonas and Klebsiella species isolates in Lagos University Teaching Hospital. AfricanJournal of Clinical and Experimental Microbiology 14 (3): 164-168.
Schulz, M., Gill, J., Zubari, S., Huber, R. and Gordon, F. (2003). Bacteriological contamination of computer keyboards in a Teaching Hospital. Infection Control Hospital Epidemiology 24(4):302-303.
Selim, H. and Abaza, A. (2015). Microbial contamination of mobile phones in a health care setting in Alexandria, Egypt. GMS Hygiene and Infection Control 10:3-10.
Sheikh, A., Afsheen, A. and Sadia, K. (2003). Plasmid borne antibiotic resistance factors among indigenous Klebsiella. Canadian Journal of Botany 35 (2):243-248.
Soto, R., Chu, L., Goldman, J., Rampil, I. and Ruskin, K. (2006). Communication in critical care environments: mobile telephones improve patient care. Anesthesia and Analgesia 102 (2): 535-541.
Srikanth, P., Lakshmanan, A., Umameheswari, J. and Ezilarasanrajaram, S. (2009). Mobile phones in a tropical setting - emerging threat for infection control. Sri Ramachandaira Journal of Medicine2:18-20.
Tagoe, D., Gyande, V., and Ansah E. (2011). Bacterial contaminant of mobile phones. Central Microbiology Journal 65: 121-125.
Tambe, N. N. and Pai, C. (2012). A study of microbial floral and mrsa harboured by mobile phones of health personnel. International Journal of Recent Trends in Science and Technology 4(1):14-18.
Trevors, J.T. (1986). Plasmid curing bacteria. FEMS Microbiology Reviews 1(3-4):149-157.
Weinstein, R. A. and Hota, B. (2004). Contamination, disinfection and crosscolonisation: are hospital surfaces reservoirs for nosocomial infection? Clinical Infectious Diseases 39(8):1182-1189