Crude oil hydrocarbon degradation efficiency of indigenous bacterial strains isolated from contaminated sites in Nigeria
Article Sidebar
Main Article Content
Abstract
The crude oil degradation potential of bacterial isolates from three contaminated sites in Nigeria were investigated. Seven bacterial isolates namely Pseudomonas aeruginosa strain W15, Pseudomonas aeruginosa strain N3R, Serratia marcescens strain N4, Providencia vermicola strain W8, Serratia marcescens strain W13, Pseudomonas aeruginosa strain W11 and Pseudomonas protegens strain P7 were isolated and identified using molecular methods. Isolates N4, N3 and W13 showed higher % total petroleum hydrocarbon (TPH) degradation of 79.26%, 78.96% and 78.69% respectively than W15, P7, W8 and W11 with % TPH
degradation of 68.96%, 62.14%, 59.75% and 59.00% respectively. W13 showed the fastest degradation rate with 78.72% within the first 14 days of incubation; however, after the 14th day, there was no progressive change in % TPH. W11 showed degradation of wider range of hydrocarbon components originally in the crude oil as well as the complete degradation of most intermediates formed. The isolates showed good degradation potentials for bioremediation applications.
Downloads
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
References
Afshar-Mohajer, N., Fox, M.A., Koehler, K. (2019). The human health risk estimation of inhaled oil spill emissions with and without adding dispersant. Science of Total Environment, 654: 924-32.
Alsaffar, Z., Pearman, J.K., Curdia, J., Ellis, J., Calleja, M.L., Ruiz-Compean, P., Roth, F., Villalobos, R., Jones, B.H., Moran, X.A., Carvalho, S. (2020). The role of seagrass vegetation and local environmental conditions in shaping benthic bacterial and macroinvertebrate communities in a tropical coastal lagoon. Science Reports, 10(1): 1-7.
Azamjon, B.S., Kakushi, H. and Keiichi, E. (2011). Bioactive pigments from marine bacteria: Applications and physiological roles. Evidence-based complementary and Alternative Medicine, Article ID670349, 17 pages.doi.org/10.1155/2011/670349.
Bhagobaty, R.K. (2020). Hydrocarbon-utilizing bacteria of natural crude oil seepages, Digboi oilfield, Northeastern region of India. Journal of Sedimentary Environment, 5(2): 177-85.
Boopathy, R. (2000). Factors limiting bioremediation technologies.Bioresource Technology. 7: 63 – 67.
Bushnell, L.D. and Haas, H.F. (1941). The utilization of hydrocarbons by microorganisms. Journal of Bacteriology, 41: 653 – 673.
Camacho-Montealegre, C.M., Rodrigues, E.M., Morais, D.K., Tótola, M.R. (2021). Prokaryotic community diversity during bioremediation of crude oil contaminated oilfield soil: effects of hydrocarbon concentration and salinity. Brazilian Journal of Microbiology, 52(2): 787-800.
Cerqueira, V.S., Hollenbach, E.B., Maboni, F., Vainstein, M.H., Camargo, F.A., do Carmo, R.P.M. and Bento, F.M. (2011). Biodegradation potential of oily sludge by pure and mixed bacterial cultures.Bioresource Technology, 102(23): 11003 – 11010.
Chaillan, F., Le-Fleche, A., Bury, E., Phantavong, Y., Grimont, P., Saliot, A. and Oudot, J. (2006). Identification and biodegradation potential of tropical aerobic hydrocarbondegrading microorganism. Resource Microbiology, 155: 587 – 595.
Chikere, C.B., Azubuike, C.C. and Etefia, E.E. (2017). Biodegradation potential of indigenous bacteria isolated from a crude oil polluted soil. Journal of Environmental and Biotechnology Research, 6(2): 213 –219.
Chen, Q., Li, J., Liu, M., Sun, H. and Bao, M. (2017). Study on the biodegradation of crude oil by free and immobilized bacterial consortium in marine environment. PLoS ONE, 12(3): e0174445.doi.org/10.1371/journal.pone.0174445.
Dasgupta, D., Gosh, R. and Sengupta, T.K. (2013). Biofilm mediated enhanced crude oil degradation by newly isolated Pseudomonas Species. Biotechnology.pp 1-13. doi.org/10.5402/2013/250749.
Dejvakumari, M., Sajivkumar, M., Suganya, A.M., RubanPrabakaran, J., Palavesam, A. and Immanuel, G. (2020). Studies on reclamation of crude oil polluted soil by biosurfactant producing Pseudomonas aeruginosa (DKB1). Biocatalysis and Agricultural Biotechnology, 29.
doi.org/10.1016/j.bcab.2020.101773.
Dhaegheem, H.N., Jazza, S.H., Aziz, Z.S. (2021). Biochemical, molecular and ecological characterization of petroleum biodegradable bacteria in Misan Province/Iraq. Journal of Chemical Health Risks. 11. doi.org/10.22034/jcgr.2021.686884.
Hanafy, A.A.M., Yasir, A., Mohamed, S.A., AlGarni, S.M.S., Sabir, J.S.M. and AbuZinadah, O.A. (2015). Isolation and identification of bacterial consortia responsible for degrading oil spills from the coastal area of Yanbu, Saudi Arabia.Biotechnology and Biotechnological
equipment. 30(1): 69-74
Holt, S.G., Krieg, N.R., Sneat, P.H.A., Stanley, J.T. and William, S.T. (1994). Bergey’s Manual of Determinative Bacteriology.Wlliams and Wilikins, Baltimore. pp 786-788.
Horowitz, A., Gutnick, D. and Rosenberg, E. (1975). Sequential growth of bacteria on crude oil. Applied Microbiology. 30: 10 -19.
Ite, A.E., Harry, T.A., Obadimu, C.O., Asuaiko, E.R., Inim, I.J. (2018). Petroleum hydrocarbons contamination of surface water and groundwater in the Niger Delta region of Nigeria. Journal of Environmental Pollution and Human Health. 6(2): 51-61.
Kaczorek, E. and Olszanowski, A. (2011). Uptake of hydrocarbon by Pseudomonasfluorescens (P1) and Pseudomonasputida (K1) strains in the presence of surfactants: A cell surface modification.Water Air Soil Pollution. 214(1-4): 452 –459.
Kertesz, M.A., Schmidt-Larbig, K. and Wuest, T. (1999). A novel reduced flavin mononucleotide-dependent methane sulfonate sulfonatase encoded by the sulfur-regulated msu operon of Pseudomonas aeruginosa. Journal of Bacteriology. 181: 146 – 1473.
Koma, D., Hasumi, S. and Motoki, K. (2003). Biodegradation of n-alkylcyclohexanes by co-oxidation via multiple pathways in Acinetobacter sp. ODDK71. Journal of Bioscience and Bioengineering. 95: 641 -644.
Liu, Y., Yang, Y., Chunjiang, W., Ma, Z., Liu, X.and Li, S. (2020). Biodegradation of nalkanes in crude oil by three identified bacterial strains. Fuel. 275.doi.org/10.1016/j.fuel.2020.117897.
Malatova, K. (2005). Isolation and characterisation of hydrocarbon degrading bacteria from environmental habitats in western New York State. M.Sc. thesis, Institute of Technology, Rochester. pp 1 – 108.
Michaud, L., Lo Giudice, A., Saitta, M. and De Domenico, V.M. (2004). The biodegradation efficiency on diesel oil by two psychrotrophic antarctic marine bacteria during a two-month-long experiment. Marine Resource Bulletin, 49:405–409.
Michel, J. and Fingas, M. (2015). Oil spills: causes, consequences, prevention and countermeasures. Fossil fuels: current
status and future directions. pp 159– 201.doi.org/10.1142/9789814699983_0007.
Moss L. (2010). The 13 largest oil spills in history. Mother Nature Network . http://www.mnn.com/earthmatters/wildernessresources/stories/the13-largest-oil-spills-in-history, (Accessed August, 2019).
Motta, F.L., Stoyanov, S.R., Soares, B.P. (2018). Application of solidifiers for oil spill containment: A review. Chemosphere, 194: 837 – 846.
Naik, M.G. and Duraphe, M.D. (2012). Review paper on - Parameters affecting bioremediation. International journal of Life Sciences and Pharma Research,2(3): 77 – 80.
Nnabuife, O.O., Ogbonna, J.C., Anyanwu, C., Ike, A.C. (2021). Population dynamics and crude oil degrading ability of bacterial consortia of isolates from oilcontaminated sites in Nigeria. International Microbiology, 22: 1-3.
Nwilo, P.C. and Badejo, O.T. (2006). Impacts and management of oil spill pollution along the Nigerian coastal areas. In: Peter C, Administering Marine Spaces: International Issues. Published by the International Federation of Surveyors (FIG), Denmark. ISBN: 87-90907-55-8.
Obi, L.U., Atagana, H.I. and Adeleke, R.A.(2016). Isolation and characterisation of crude oil sludge bacteria. Springer Plus5: pp.1946. doi.org/10.1186/s40064-016-3617-z.
Ogbonna, J.C., Yoshizawa, H. and Tanaka, H. (2000). Treatment of high strength organic wastewater by a mixed culture of photosynthetic microorganisms. Journal of Applied Phycology, 12(3-5): 277 – 284.
Paliwal, V., Puranik, S. and Purohit, H.J. (2011). Integrated perspective for effective bioremediation. Applied Biochemistry and Biotechnology, 66:903–924.
Pawar, R.M. (2015). The effect of soil pH on degradation of polycyclic aromatic hydrocarbons (PAHs). Journal of Bioremediation and Biodegradation, 6:291. doi: 10.4172/2155-6199.1000291.
Ra, T., Zhao, Y., Zheng, M. (2019). Comparative study on the petroleum crude oil degradation potential of microbes from petroleum-contaminated soil and noncontaminated soil. International Journal of Environmental Science and Technology, 16(11): 7127-36.
Rajasekar, A., Babu, T.G., Pandian, S.T.K., Maruthamuthu, S., Palaniswamy, N. and Rajendran, A. (2007). Role of Serratia marcescens ACE2 on diesel degradation and its influence on corrosion. Journal of Industrial Microbiology and Biotechnology, 34: 589 – 598. doi: 10.1007/s10295-007-0225-5.
Ridgway, H.F., Safarik, J., Phipps, D., Carl, P.and Clark, D. (1990). Identification and catabolic activity of well-derived gasoline-degrading bacteria from a contaminated aquifer. Applied and Environmental Microbiology,56(11): 3565–3575.
Shukla, A. and Cameotra, S.S. (2012). Hydrocarbon pollution: Effects on living organisms, remediation of contaminated environments, and effects of heavy contaminants co-contamination on bioremediation. In: Dr. Laura ReomeroZeron (Ed.), Introduction to enhanced oil
recovery (EOR) processes and bioremediation of oil-contaminated sites. ISBN: 978-953-51-0629-6, InTech. Pp 186-206.
Uba, B.O., Chukwura, E.I., Okoye, E.L., Ubani, O., Irabor, M.I., Onyekwuluje, N.V., Ajeh, J.E., Mmuogbo, C.S., Nwafor, M.C., Igboesorom, C.C., Nwodo, C.J. (2018). Multiple degradation and resistance capabilities of marine bacteria isolated from Niger Delta, Nigeria on petroleum
pollutants and heavy metals. Journal of Advanced Biology and Biotechnology, 20(1): 1-7.
United Nations Environmental Programme (2017). UNEP Ogoni land oil assessment reveals extent of environmental contamination and proffered clean-up recommendations. Retrieved from http://www.unenvironment.org/newsand-stories/story/unepogoniland -oil-.
(Accessed October, 2019).
Varjani, S.J., Rana, D.P., Jain, A.K., Bateja, S. and Upasani, V.N. (2017). Microbial dynamics in petroleum oilfields and their relationship with physiological properties of petroleum oil reservoirs. Bioresource Technology, 245: 1258 –1265
Wackett, L.P. (2003). Pseudomonas putida: A versatile biocatalyst. National Biotechnology,21:136–138.
Wang, Q., Zhang, S., Li, Y. and Klassen, W. (2011). Potential approaches to improving biodegradation of hydrocarbons for bioremediation of crude oil pollution. Journal of Environmental Protection, 2(1): 47– 55.
Wang, X., Cai, T., Wen, W., Ai, J., Ai, J., Zhang, Z., Zhu, L. and George, S.C. (2020).Surfactin for enhanced removal of aromatic hydrocarbons during biodegradation of crude oil. Fuel. pp 267.doi.org/10.1016/j.fuel.2020.117272.
Wartell, B., Boufadel, M., Rodriguez-Freire, L. (2021). An effort to understand and improve the anaerobic biodegradation of petroleum hydrocarbons: A literature review. International Biodeterioration and Biodegradation. 157: pp 105156.
Wongsa, P., Tanaka, M., Ueno, A., Hasanuzzaman, M., Yumoto, I. and Okuya (2004). Isolation and characterisation of novel strains of
Pseudomonas aeruginosa and Serratiamarcescens possessing high efficiency to degrade gasoline, kerosene, diesel oil, and lubricating oil. Current Microbiology,49(6): 415 – 22.
Xia, M., Liu, Y., Taylor, A.A., Fu, D., Khan, A.R. and Terry, N. (2017). Crude oil depletion by bacterial strains isolated from a petroleum hydrocarbon impacted solid waste management site in California.International Biodeterioration and Biodegradation, 123: 70 – 77.
Xu, X., Liu, W., Tian, S., Wang, W., Qi, Q., Jiang, P., Gao, X., Li, F., Li, H. and Yu, H. (2018). Petroleum hydrocarbondegrading bacteria for the remediation of oil pollution under aerobic conditions: A perspective analysis. Frontiers in Microbiology,9:2885. doi.org/10.3389/fmicb.2018.02885.
Xu, X., Zhai, Z., Li, H., Wang, Q., Han, X. and Yu, H. (2017). Synergetic effect of biophotocatalytic hybrid system: g-C3N4, and Acinetobacter sp. JLS1 for enhanced degradation of C16 alkane. Journal of Chemical Engineering. 323: 520 – 529.