Assessing microbial exoelectrogenicity for enhanced industrial waste water management and power generation
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Abstract
The quest for eco-friendly waste management and renewable sources of energy is rapidly increasing. Microbial fuel cell is promising form of renewable energy, which treat and convert organic matter in wastewater to electricity through the aid of microorganisms present in the wastewater. This study assessed for electrogenic microorganisms that generate power during wastewater treatment at a referenced pH of 8.5 and temperature of 37 0C. The exoelectrogenicity and identity of the microbial isolates were confirmed using
microbial fuel cell (MFC) and molecular characterization, respectively. Two bacterial isolates: N4- Providencia species, N6- Proteus species, and three fungal isolates: S9- Clavispora lusitaniae, S10- Candida parapsilosis ,S14- Clavispora lusitaniae with accession numbers; KX548357.1, KX548358.1 and KX548359.1, KX548360.1, KX548361.1, respectively showed exoelectrogenic properties. Proteus species and Candida parapsilosis generated relative high-power densities of 1.59 and 1.55 W/m2, respectively. Significant difference (p < 0.05) in wastewater treatment was also observed. When compared with the control wastewater, S10 recorded about 38% of contaminant removal with the following parameters; biochemical oxygen demand (536.38mg/l), chemical oxygen demand (1974mg/l), total dissolved solid (640mg/l) and conductivity (512µS/cm). The findings showed that, not only bacteria, but fungi are good exoelectrogenic microorganisms for industrial wastewater treatment and power generation in MFC setup.
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References
Altschul, S.F., Gish, W., Miller, W., Myers, E.W. and Lipman, D.J. (1990). Basic local alignment search tool. Journal of Molecular Biology, 215: 403-410.
Ang, T., Salem, M., Kamarol, M., Das, H.S., Nazari, M.A. and Prabaharan, N. A. (2022). Comprehensive study of renewable energy sources:
Classifications, challenges and suggestions. Energy Strategy Reviews, 43: 2211-467. https://doi.org/10.1016/j.esr.2022.100939.
Behera, M., Murthy, S.S. and Ghangrekar, M.M. (2011). Effect of operating temperature on performance of microbial fuel cell. Water Science &Technology, 64: 917-922.
Cheng, S. and Logan, B.E. (2011). Increasing power generation for scaling up single chamber air cathode microbial fuel cells. Bioresource Technology, 102: 4468-4473.
Das, M.P. (2015). Bioelectricity production using algae in microbial fuel cell. Der Pharma Chemica, 7(11): 8-10.
De La Cruz-Noriega, M., Benites, S.M., RojasFlores, S., Otiniano, N.M., Sabogal Vargas, A.M. and Alfaro, R. (2023). Use of wastewater and electrogenic bacteria to generate eco-friendly electricity through microbial fuel cells. Sustainability, 15:10640. https://doi.org/10.3390/su151310640
Hall, T. A. (1999). BioEdit: a user friendly biological sequence alignment editor and analysis program for Windows 95/98/NT, Nucleic Acids Symposium Series, 41: 95-98.
Indian Standard 3025. (2003). Method of sampling and test (Physical and Chemical) for water and wastewater: Biochemical oxygen demand. Bureau of Indian Standard: Part 44.
Indian Standard 3025. (2013). Method of sampling and test (physical and chemical) for water and wastewater: Filterable residue and non-filterable residue (total suspended solid and total dissolved solid). Bureau of IndianStandard: Part 16 and 17. Indian Standard 3025. (2006). Method of sampling and test (physical and chemical) for water and wastewater: Chemical oxygen demand, Bureau of Indian Standard: Part 58.
Kabeyi, M. J. B. and Olanrewaju, O. A. (2022). Sustainable energy transition for renewable and low carbon grid electricity generation and supply. Frontiers in Energy Research, 9:74-3114. DOI=10.3389/fenrg.2021.743114.
Lai, M.F., Lou, C.W. and Lin, J.H. (2018). Improve3D electrode materials performance on electricity generation from livestock wastewater in microbial fuel cell. International Journal of Hydrogen Energy, 43: 11520–11529.
Liu, X., Ueki, T. and Gao, H. (2022). Microbial biofilms for electricity generation from water evaporation and power to wearables. Nature Communications, 13:4369 https://doi.org/10.1038/s41467-022-32105-6
Logan, B.E., Wallack, M.J., Kim, K.Y., He, W., Feng, Y. and Saikaly, P. (2015). Assessment of microbial fuel cell configurations and power densities.
Environmental Science Technology Letters, 22 (8): 206-214.
Logan, B. E., Rossi, R., Ragab, A. and Saikaly, P. E. (2019). Electroactive microorganisms in bioelectrochemical systems. Nature Reviews Microbiology, 17: 307–319.
Madigan, M. T. (2012). Brock Biology of Microorganisms. 13th ed. San Francisco: Benjamin Cummings, 22-20.
Mouhib, M., Reggente, M., Li, L., Schuergers, N. and Boghossian, A.A. (2023). Extracellular electron transfer pathways to enhance the electroactivity of modified Escherichia coli. Joule, 7 (9): 2092-2106.
Nwagu, K. E., Ekpo, I. A., Ekaluo, B. U., Ubi, G. M., Elemba, M. O. and Uzoh, C. V. (2019). Optimization and Molecular Characterization of Exoelectrogenic Isolates for Enhanced Microbial Fuel Cell Performance. Microbiology and Biotechnology Letters, 47(4): 621-629.
Osman, M. H., Shah, A. A. and Walsh, F. C. (2010). Recent progress and continuing challenges in bio fuel cells. Part II: Microbial. Biosensors& Bioelectronics, 26: 953-963.
Padma, S. and Dirk, B.H. (2012). Microbial fuel cells: Future fuel technologies. National Petroleum Council (NPC) Study, 13:23.
Park, T., Ding, W., Chend, S., Brar, M.S., Ma, A.P., Tun, H.M. and Leung, F.C. (2014). Microbial community in microbial fuel cell (MFC) medium and effluent enriched with purple photosynthetic bacterium (Rhodopseudomonas sp). Journal of Applied Microbiology, 4:22.
Roy, H., Rahman, T.U., Tasnim, N., Arju, J., Rafid, M.M. and Islam, M.R. (2023). Microbial fuel cell construction features and application for sustainable wastewater treatment. Membranes (Basel). 13(5):490. doi: 10.3390/membranes13050490.
Sebastià, P., Marc, S., Marta, C., Marina, C., Dolors, M.B. and Jesús, C. (2010). Effect of pH on nutrient dynamics and electricity production using microbialfuel cells. Bioresource Technology,101: 9594-9599.
Sekrecka-Belniak, A. and ToczyłowskaMamińska, R. (2018). Fungi-based microbial fuel cells. Energies, 11:2827. https://doi.org/10.3390/en11102827.
Watson, V.J. and Logan, B.E. (2010). Power production in MFCs inoculated with Shewanella oneidensis MR-1 or mixed cultures. Biotechnology and
Bioengineering, 105: 489-498.
Weisburg, W.G., Barns, S.M., Pelletier, D.A. and Lane, D.J. (1991). 16S ribosomal DNA amplification for phylogenetic study. Journal of Bacteriology, 173: 697-703.
White, T.J., Bruns, T., Lee, S. and Taylor, S. (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Inni, M.A., Gelfand, D.H., Sninsky, J.J., White, T.J (eds.), PCR Protocols: a guide to methods and applications. New York, NY: Academic Press. (pp. 315-322).
Wolfson, R. (2012). High-Energy Society, in Energy. Environment and Climate, 2nd ed. New York, U.S.A.: Norton, 2012, pp. 20–21
Xu, B., Ge, Z. and He, Z. (2015). “Sediment microbial fuel cells for wastewater treatment: Challenges and opportunities in environmental science. Water Resource Technology, 10:10-39.
Yu, F., Qiu, F. and Meza, J. (2016). 12 - Design and statistical analysis of massspectrometry-based quantitative proteomics data. Editor(s): P.
Ciborowski, J. Silberring, Proteomic Profiling and Analytical Chemistry (Second Edition), Elsevier, 2016, Pages 211-237. https://doi.org/10.1016/B978-0-444-63688-1.00012-4.
Zuo, Y., Maness, P.C. and Logan, B.E. (2008). Electricity production from steam exploded corn stover biomass. Energy Fuels, 20: 1716-1721.