Evaluation of Larvicidal Activity of Leaf Extract of Lantana camara (Family: Verbenaceae) Against the Aedes aegypti Mosquito
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Abstract
Aqueous and ethanolic leaf extracts of Lantana camara were prepared and four different concentrations (0.3, 0.6, 0.9 and 1.2 g/ l) of both extracts were tested for larvicidal activity against the fourth instar larvae of Aedes aegypti for 24 hours in the laboratory. The 24-h percentage mortality rates ranged from 91.66 to 96.66 % in larvae treated with 0.3 to 1.2 g/L ethanolic L. camara leaf extract, respectively. The 24-h lethal time (LT50) and lethal concentration (LC50) were 6.8 h and 0.48 g/l, respectively. The aqueous extract did not result in
more than 35 % larval mortality. The 24-h percentage mortality between the treatment groups were significantly different (P < 0.001) and also when compared with the control (P < 0.05). The result showed that the ethanolic extract was more lethal than the aqueous extract to A. aegypti larvae. The ethanolic extract also limited the pupation and the adult emergence that was inhibited by the ethanolic extract at 0.9 and 1.2g/L extract.
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References
Bishnu RJ, and Wiesman, Z (2005). Larvicidal
effects of aqueous extracts of Balaites
aegyptiaca ( desert date) against the
larvae of Culex pipens mosquitoes Afr.
J. Biotechnol. 4(11): 1351-1354
Bor, NL (1953). Manuel of Indian forest
Botany. First Edition, Oxford
University press London 79 pp.
Bouda, H, Tapondjou, L. A, Fontem, D A and
Gumedzoe , MYD (2001) Effect of
essential oils fom leaves of Ageratum
conyzoides, Lantana camara and
Chromolaena odorata on the mortality
of Sitophilus zeamais ( Coloeptera:
Curculionidae). J. Stored Prod. Res.
: 103-109
Brown, AWA (1986). Insecticide resistance in
mosquitoes: a pragmatic review. J.
Am. Mosq. Control. Assoc. 2: 123 –
Cheng, SS, Clang, HT, Chang, ST, Tsai, KH
and Cheng WJ. (2003) Bioactivity of
selected plant essiential oils against
the yellow fever mosquito Aedes
aegypti larvae. Bioresourse Technol..
: 99 – 102.
Dwivedi SC and Garg, S (2003). Toxicity evaluation of flower extracts of Lantana camara on the life cycle of Corcyra cephalonica. Indian J. Entomol 65: 330-334
Finney, DJ (1971). Probit analysis. University Press. Cambridge. United Kingdom.
Gubler, D J (1997). Epidemic dengue, dengue haemorhagic fever: A global public health problem in the 21st Century. Dengue Bulletin 2: 21 – 31.
Harborne, JB (1973) Phytochemical methods. First edition. London, Chapman and Hall Ltd. pp 49-188
Harre G and Kamath V (2004).Larvicidal activity of plant extracts used alone and in combination with known synthetic larvicidal agents against
Aedes aegypti. Indian J. Exp. Biol. 42: 1216-1219
Hayes JB. and Laws ER (1991).Handbook of pesticide toxicology Vol. I . Academic. San Diego. C A.
Jackal LEN (1993).The use of neem in controlling cowpea pests. International Inst. Trop. Agric. Res. Bull. 7: 5-11
Jacobson M (1981).Neem Research. in the US. Dept of Agric Clinical, biological and cultural control aspects Pg 33 – 42. In Schemutterr, M and Ascher K R. S (eds). Natural pesticides from the neem tree (Azadirachtin indica A Juss) and other tropical plants. Proceeding of the 1st International Neem Conference. Raulscholzhausen, Fed Rep of Germany, 16 – 18 years 1980.
Jang, YS, Jeon JH and Lee, HS (2005).Mosquito larvicidal activity of active constituents derived from Chamaecyparis obtuse leaves against
three mosquito species. J. Am. Mosq. Control Ass 21(4): 400 – 403.
Khanna, VG and Kannabiran K (2007) Larvicidal effect of Hemdesmus indicus, Ggymnema sylvestre and Eclipta prostrata against Culex
quinquifaciatus mosquito larvae. African J. Biotechnol. 6(3): 307-311
Khanuja SP, Kumar S, Shasany AK, Dhawan S, Darokar M.P, Tripatty AK, Satapatty S, Southa TR, Gupth VK, Pripathi AK, Awsthi S, Prajapati V, Naqri AA, Agrawal KK, Babal JR, Singh AK, Ahmed A, Bansal RP, Krishna A and Saikia D (2001). Multi-utility plant “Ganga” of Mentha spicata Var. Viridis. J. Med. Arom. Plant Sc. 23: 113 – 116.
Kodwara JO (1976) Medicinal plants of East Africa. First edition. East African literature Bureau. Kenya. 223 pp.
Lee HS.(2006) Mosquito larvicidal activity of aromatic medicinal plant oils against Aedes aegypti and Culex pipiens pallens J. Am. Mosq. Control Assoc. 22(2): 292-295
Liu W, Zhang L, Jamel HH, Juliana J, Zailini H and Bernard GD (2003) Mosquitoe coil emissions and health implications. Envir. Health Persp. 111 (12): 1430-1439.
Minijas JN and Sarda RK (1986) Laboratory observation on the toxicity of Swrtzia madagascariensis (Leguminosae) extract on mosquito larvae. Trans of Royal Soc. Trop. Med. Hyg. 80: 460 – 461.
Mohammed A, Chadee DD (2007) An evaluation of some Trinidadian plant extracts against larvae of Aedes aegypti mosquitoe. J Am. Mosq.
Control Assoc. 23 (2): 172-176
Mohan DR , Ramaswamy M(2007). Evaluation of larvicidal activity of the leaf extract of a weed plant, Agerantina adenophora, against two
important mosquitoes, Aedes aegypti and Culex quinquefasciatus. Afr. J. Biotechnol. 6(5): 631-638
Mohinder SJ (2001) Toxic effect of garlic extracts on the eggs of Aedes aegypti Diptera: Culinidae; A Scanning electron microscopic study. J. Med.
Entomol. 38: 446 – 450.
Mulla MS and Su T (1999) Activity and biological effects of neem product against arthropods of medical and veterinary importance. J. Am Mosq.
Control Assoc. 5: 133 – 152.
Muthukrishnan J and Pushpalatha E (2001) Effects of plant extracts on fecundity and fertility of mosquitoes. J. Appl. Entomol. 125: 31-35
Mwangi RW and Mukiama JK (1988) Evaluation of Melina volkensi extracts as mosquito larvicide J. Am. Mosq. Control Assoc. 4: 4472-447
Nasidi A, Monath T P, Deock K, Tomori O, Cordelleir R, Olaleye OD, Harry TO, Adeniyi JA, Sorungbe AO, Ajose– Coker AO, Van der Laan G and
Oyediran AB O (1989) Urban yellow fever epidemic in western Nigeria, Trans. Royal Soc. Tropical Med. Hygene 83 (3): 401 – 406:
Ndione RD, Faye,O, Ndiaye M, Abdoulaye D and Afoutou JM(2007). Toxic effect of neem products (Azadirachta indica A. Juss) on Aedes
aegypti Linnaeus 1762 larvae. Afr. J. Biotechnol. 6(24): 2846-2854.
Rao DR, Reuben R, Venugopal MS, Nagasampagi BA and Schutterer H (1992) Evaluation of neem, Aadirachta indica, with or without water
management, for the control of culicine mosquito larvae in rice fields. J Med. Vet. Entomol 6(4): 318-324.