Production of fuel ethanol from Vitellaria paradoxa fruit pulp by simultaneous saccharification and fermentation

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Abdul-Mumeen Idrissu
Zakpaa Hilary Domakyaraa
Mills-Robertson Felix Charles
Samuel Tetteh Lowor

Abstract

Biofuels have become the world’s greatest, safest, cleanest and cheapest alternative to fossil fuels. Transport sector continues to receive influx of cars and general machinery every year worldwide leading to an overwhelming use of fossil fuel. The fossil fuel sources are gradually becoming depleted. Sustainable bioethanol recovered from inexhaustible sources are constantly been used to replace fossil fuels in the transport sector. The shea nut pulp (SNP) is a cheap source of carbon, nitrogen and calcium for the production of bioethanol. This study has employed the shea nut pulp as substrate, Saccharomyces cerevisiae-the Baker’s Yeast strain-as the fermentation microbe, Alpha Amylase enzyme for hydrolysis of the substrate and supported the simultaneous fermentation process with nutrient supplements. The data were subjected to statistical analysis. Approximately 37.8 and 40.0 g/L of ethanol after 16 and 20 h, were produced from without-α-amylase and with-α-amylase treatments, respectively. Fermentation efficiencies of 58.6 and 65.0 % were observed ethanol yields for without-α-amylase and with-α-amylase, respectively when compared to the theoretical yield. This research is useful in harnessing the potentials of the shea nut pulp as industrially relevant substrate for use independently or in combination with other substrates in microbial fermentation processes for ethanol production. An assessment of the fermentation process has revealed that the SNP is a good source of bioethanol going into the future. The research therefore recommends further work on scale-up of the bioethanol production process from the shea nut pulp.

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How to Cite
Idrissu, A.-M., Domakyaraa, Z. H., Charles, M.-R. F., & Lowor, S. T. (2023). Production of fuel ethanol from Vitellaria paradoxa fruit pulp by simultaneous saccharification and fermentation. Journal of Biological Research and Biotechnology, 21(2), 1935-1951. https://doi.org/10.4314/br.v21i2.2
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References

Abd-Rahim, F., Wasoh, H., Zakaria, M. R., Ariff, A., Kapri, R., Ramli, N., and Siew-Ling, L. (2014). Production of high yield sugars from Kappaphycus alvarezii using combined methods of chemical and enzymatic hydrolysis. Food Hydrocolloids, 42: 309 - 315.

Abdul-Mumeen, I, Marcel, T. A., Anders, T., Moses, M. Y., and Anne, M. S. (2016). Hydrolysis and fermentation of Ghanaian green seaweeds for bioethanol production. Innovation Conference Ghana: Development innovation-putting the pieces together, La Palm Royal Beach Hotel, Accra, Ghana. pp 261 - 281.

Alzeer, J., and Abou Hadeed, K. (2016). Ethanol and its Halal status in food industries. Trends in Food Science & Technology, 58: 14 - 20.

Amos, J. (2018). The fermentation of fructose in winemaking, the wine expert. Lallemand Australia Pty Ltd, 23-25 Erudina Ave Edwardstown, 5039, SA [Retrieved from www.lallemandwine.com on the 10th of May, 2022].

Arino, J. (2010). Integrative responses to high pH stress in S. cerevisiae. OMICS A Journal of Integrative Biology, 14(5): 517 – 523.

Bensah, E. C., Mensah, M., and Schmidt, J. E. (2012). Implementation of 2nd generation bioethanol production in Ghana. In H. Jørgensen (Ed.), Advanced Biofuels in a Biorefinery Approach (pp. 115-115).

Berthels, N. J., Cordero Otero, R. R., Bauer, F. F., Thevelein, J. M., and Pretorious, I. S. (2004). Discrepancy in glucose and fructose utilization during fermentation by Saccharomyces cerevisiae wine yeast strains. FEMS Yeast Research, 4: 683 - 689.

Borines, M. G., de Leon, R. L., and Cuello, J. L. (2013). Bioethanol production from the macroalgae Sargassum spp. Bioresource Technology, 138: 22 - 29.

Chen, A. K. L., Gelling, C., Rogers, P. L., Dawes, I. W., and Rosche, B. (2009). Response of Saccharomyces cerevisiae to stress-free acidification. Journal of Microbiology, 47(1): 1 - 8.

Fleet, G. H. (1998). The microbiology of alcoholic beverages. In: Microbiology of fermented foods (Wood, B.J.B., Ed). Blackie, Glasgow. pp 217-262.

Gao, F., Gao, L., Zhang, D., Ye, N., Chen, S., and Li, D. (2015). Enhanced hydrolysis of Macrocystis pyrifera by integrated hydroxyl radicals and hot water pretreatment. Bioresource Technology, 179: 490 – 496.

Gladis, A., Bondesson, P. M., Galbe, M., and Zacchi, G. (2015). Influence of different SSF conditions on ethanol production from corn stover at high solids loadings. Energy Science & Engineering, 3(5); 481 - 489.

Henderson, C. M., Lozada-Contreras, M., Jiranek, V., Longo, M. L., and Block, D. E. (2013). Ethanol production and maximum cell growth are highly correlated with membrane lipid composition during fermentation as determined by lipidomic analysis of 22 Saccharomyces cerevisiae strains. Applied and Environmental Microbiology, 79(1): 91 - 104.

IPCC (2007). Climate Change 2007: An assessment of the intergovernmental panel on climate change. Geneva, Switzerland.

Kádár, Szengyel and Réczey, K. (2004). Simultaneous saccharification and fermentation (SSF) of industrial wastes for the production of ethanol. Industrial Crops and Products, 20: 103 - 110.

Karray, R., Hamza, M., and Sayadi, S. (2015). Evaluation of ultrasonic, acid, thermo- alkaline and enzymatic pre-treatments on anaerobic digestion of Ulva rigida for biogas production. Bioresource Technology, 187: 205 - 213.

Klanarong, S., Sittichoke, W., and Kuakoon, P. (2012). Cassava bioethanol. Cassava and Starch Technology Research Unit, National Center for Genetic Engineering and Biotechnology (BIOTEC) Thailand.

Kostas, E. T., White, D. A., and Cook, D. J. (2020). Bioethanol production from UK seaweeds: investigating variable pre- treatment and enzyme hydrolysis parameters. Bioenergy Research, 13(1): 271 - 285.

Lee, H. J., Kim, S. J., Yoon, J. J., Kim, K. H., Seo, J. H., and Park, Y. C. (2015). Evolutionary engineering of Saccharomyces cerevisiae for efficient conversion of red algal bio sugars to bioethanol. Bioresource Technology, 191: 445 - 451.

National Research Council (1999). Review of the research strategy for biomass-derived transportation fuels. National Academies Press.

OECD/FAO (2017). Market situation 15 - 18. Retrieved from https://www.fao.org/3/BT098e/BT098e.p df. Accessed January 20, 2023

Ofosu-Appiah, C., Zakpaa, H. D., Mak-Mensah, E., and Bentil, J. A. (2016). Evaluation of ethanol production from pito mash using Zymomonas mobilis and Saccharomyces cerevisiae. African Journal of Biotechnology, 15(30): 1613-1620.

Ojo, O. A., and Adebayo, T. A. (2013). Bio- deterioration of shea butter fruit (Vitellaria paradoxa) in storage and its effects on the nutrient composition. Report and Opinion, 5 (12): 13 - 18.

Paul, H. (2010). Monitoring growth of beer brewing strains of Saccharomyces cerevisiae. Application Note; BioTek Instruments, Inc., Winooski, VT, pp 1 - 6.

Perera, F. (2018). Pollution from fossil-fuel combustion is the leading environmental threat to global pediatric health and equity: Solutions exist. International Journal of Environmental Research and Public Health, 15(1): 16.

Puspawati, S., Ainuri, M., and Nugraha, D. A. (2015). The production of bioethanol fermentation substrate from Eucheuma cottonii seaweed through hydrolysis by cellulose enzyme. Agriculture and Agricultural Science Procedia, 3: 200 -205.

Renewable Fuels Association (RFA). (2018). Ethanol strong: 2018 ethanol industry outlook, 1-19. Retrieved from http://www.ethanolrfa.org/wp- content/uploads/ 2018/02/NECfinalOutlook.pdf. Assessed November 11, 2022.

Roca-Mesa, H., Sendra, S., Mas, A., Beltran, G., and Torija, M. J. (2020). Nitrogen preferences during alcoholic fermentation of different non-Saccharomyces yeasts of oenological interest. Microorganisms, 8(2): 157.

Schultz-Jensen, N., Thygesen, A., Leipold, F., Thomsen, S. T., Roslander, C., Lilholt, H., and Bjerre, A. B. (2013). Pretreatment of the macroalgae Chaetomorpha linum for the production of bioethanol–comparison of five pretreatment technologies. Bioresource

Technology, 140: 36 - 42.

Suhas, V. B, Arun P, Naveen, S. H. G., and Ashok, K. (2013). Production of bioethanol from fruit rinds by saccharification and fermentation. International Journal of Scientific Research Engineering and Technology (IJSRET), 2: 362 – 365.

Thomsen, A. B., Medina, C., and Ahring, B. K. (2003). Biotechnology in ethanol production. In Risø energy report 2. New and emerging bioenergy technologies.

Thygesen, A., Marzorati, M., Boon, N., Thomsen, A. B., and Verstraete, W. (2011). Upgrading of straw hydrolysate for production of hydrogen and phenols in a microbial electrolysis cell (MEC). Applied Microbiology and Biotechnology, 89(3): 855 - 865.

Tomás, A. F. (2013). Optimization of bioethanol production from carbohydrate rich wastes by extreme thermophilic microorganisms (Doctoral Dissertation). Technical University of Denmark, 2013.

Trivedi, P., Delgado-Baquerizo, M., Trivedi, C., Hu, H., Anderson, I. C., Jeffries, T. C., and Singh, B. K. (2016). Microbial regulation of the soil carbon cycle: evidence from gene– enzyme relationships. The International Society for Microbial Ecology, 10(11): 2593 - 2604.

Tronchoni, J., Gamero, A., Arroyo-López, F. N., Barrio, E., and Querol, A. (2009). Differences in the glucose and fructose consumption profiles in diverse Saccharomyces wine species and their hybrids during grape juice fermentation. International Journal of Food Microbiology, 134 (3): 237 - 243.

Van der Wal, H., Sperber, B. L., Houweling-Tan, B., Bakker, R. R., Brandenburg, W., and López-Contreras, A. M. (2013). Production of acetone, butanol, and ethanol from biomass of the green seaweed Ulva lactuca. Bioresource Technology, 128: 431 - 437.

Wadi, A., Ahmad, A., Tompo, M., Hasyim, H., Tuwo, A., Nakajima, M., and Karim, H. (2019). Production of bioethanol from seaweed, Gracilaria verrucosa and Eucheuma cottonii, by simultaneous saccharification and fermentation methods. In Journal of Physics: Conference Series, 1341(3): 032031.

Watts, N., Adger, W. N., Agnolucci, P., Blackstock, J., Byass, P., Cai, W., and Costello, A. (2015). Health and climate change: policy responses to protect public health. The lancet, 386(10006): 1861-1914.

Wiratno, E. N., Ardyati, T., and Wardani, A. K. (2015). Effect of reducing sugar and total nitrogen to ethanol production from molasses by Saccharomyces cerevisiae. The Journal of Experimental Life Science, 4(2): 50 – 55.

Yazdani, P., Zamani, A., Karimi, K., and Taherzadeh, M. J. (2015). Characterization of Nizimuddinia zanardini macroalgae biomass composition and its potential for biofuel production. Bioresource Technology, 176: 196 - 202.

Yuwa-Amompitak, T. (2010). Ethanol production from cassava starch by selected fungi from tan-koji and Saccaromycetes cereviseae. Biotechnology, 9(1): 84 - 88.

Zakpaa, H. D., Mak-Mensah, E. E., and Johnson, F. S. (2010). Saccharification of maize agrowastes by cellulolytic fungi isolated from Ejura Farms in Ejura, Ghana. Journal of Science and Technology (Ghana), 30(1).

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