Evaluation of some organic substrates for the growth and yield of oyster mushroom Pleurotus ostreatus (Jacq.Fr.) Kumm in southeast Nigeria
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Abstract
Four organic substrates; Mahogany Sawdust (MSD), Corn Cobs (CC), Oil Palm Fruit Fibre (OPFF) and Rice Bran (RB) were evaluated for their effects on growth and yield of Pleurotus ostreatus (Jacq.Fr.) Kumm. The completely randomized experimental design was adopted for the study with 4 treatments replicated 10 times. Results on mean number of days for spawn run, primordial formation and formation of fruit body were 19.90±0.28(CC)-25.20±0.29(MSD), 45.10±0.28(CC)-47.90±0.23(MSD) and 56.50±0.22(CC)- 59.40±0.27(MSD), respectively. Similarly, height of stipe, diameter of stipe and diameter of pileus ranges were 3.28±0.13 (MSD)-3.51±0.06 (RB),1.26±0.06 (OPFF)-1.39±0.05(RB) and 4.08±0.05(CC)- 4.70±0.04(OPFF), respectively. Fresh weights (g), dry weights (g) and biological efficiency were
10.20±0.31(OPFF)-11.05±0.14(MSD), 3.18±0.15(CC)-3.38±0.13(RB) and 4.14±0.14 (OPPF)- 4.42±0.06(MSD), respectively. Results on mushroom growth showed that CC took the least duration for full mycelial colonization and the longest duration occurred on MSD. There were significant differences (P ≤ 0.05) between the durations required for primordia formation among the four organic substrates. The results on mushroom yield showed that mean fresh weights of harvested mushrooms varied from 10.20±0.31 g on OPFF to 11.05±0.14 g on MSD. There were no significant differences (P ≥ 0.05) in the biological efficiency of mushrooms grown on MSD, CC and OPFF substrates. Considering the growth and yield attributes RB was observed to be the best substrate and could be used for commercial production of P. ostreatus among the various organic substrates used in this study.
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References
Adjapong, O.A., Ansah, K.D., Angfaarabung, F. and Sintin, O.H. (2015). Maize residue as a viable substrate for farm scale cultivation
of oyster mushroom (Pleurotus ostreatus).Advances in Agriculture 20: 6-10.
Ahmad, N., Mahmood, F., Khalil, S.A., Zamir, R, Fazal, H. and Abbasi, B.H. (2014). Antioxidant activity via DPPH, grampositive and gram-negative antimicrobial potential in edible mushrooms. Toxicology and Industrial Health 30: 826–834.
Akinmusire, O.O., Omomowo, I.O. and Oguntoye, S.I.K. (2011). Cultivation performance of Pleurotus pulmonarius in Maiduguri, North Eastern Nigeria, using wood chippings and rice straw waste. Advances in Environmental Biology 5 (8): 2091-2094.
Anandhi, R, Annadurai, T., Anitha, T.S., Muralidharan, A.R., Najmunnisha, K., Nachiappan, V., Thomas, P.A. and Geraldine, P. (2013). Antihypercholesterolemic and antioxidative effects of an extract of the oyster mushroom, Pleurotus ostreatus, and its major constituent, chrysin, in Triton WR1339-induced hypercholesterolemic rats. Journal of Physiological Biochemistry 69: 313–323.
AOAC, (2002). Official Methods of Analysis (17th Edition). Association of Official Analytical Chemist, Maryland, 1106pp.
Arbaayah, H.H. and Kalsom, Y.U.(2013). Antioxidant properties in the oyster mushrooms (Pleurotus spp.) and split gillmushroom (Schizophyllum commune) ethanolic extracts. Mycosphere 4, 661– 673.
Buah, J.N., Vander-Puije, G.C., Bediako, E.A., Abole, E.A. and Showemimo, F. (2010) The growth and yield performance of oyster mushroom (Pleurotus ostreatus) on different substrates. Biotechnology 9: 338 - 342.
Çağlarırmak, N. (2007). The nutrients of exotic mushrooms (Lentinula edodes and Pleurotus species) and an estimated approach to the volatile compounds. Food Chemistry 105, 1188–1194.
Chiejina, N.V. and Osibe, D.A. (2015) Oil palm fruit fibre promotes the yield and quality of Lentinus squarrosulus (Mont.) Singer, an edible Nigerian mushroom. African Journal of Biotechnology 14: 1195 - 1200
Chitamba, J., Dube, F., Chiota, W.M. and Handiseni, M. (2012). Evaluation of substrate productivity and market quality of oyster mushroom (Pleurotus ostreatus) grown on different substrates. International Journal of Agricultural Research 7 (2): 100-106.
Chorváthová, V., Bobek, P., Ginter, E. and Klvanová, J. (1993). Effect of the oyster fungus on glycaemia and cholesterolaemia in rats with insulindependent diabetes. Physiological Research 42: 175–179.
Chowdhury, M.M.H., Kubra, K. and Ahmed, S.R. (2015) Screening of antimicrobial, antioxidant properties and bioactive compounds of some edible mushrooms cultivated in Bangladesh. Annals of Clinical Microbiology and Antimicrobials 14: 8–13.
Davis, R.A. and Aegeter, B. J. (2000). Edible Mushroom Cultivation. Scientific Publishers, Jodhupur, India. 143pp.
Elbatrawy, E.N., Ghonimy, E.A., Alassar, M.M. and Wu, F.S. (2015). Medicinal mushroom extracts possess differential antioxidant activity and cytotoxicity to cancer cells. International Journal of Medicinal Mushrooms 17: 471–479.
Frimpong-Manso, J., Obodai, M., Dzomeku, M. and Apertorgbor, M.M. (2011). Influence of rice husk on biological efficiency and nutrient content of Pleurotus ostreatus (Jacq. ex. Fr.) Kummer. International Food ResearchJournal 18: 249 – 254.
Goering HK and Van Soest PJ. (1970). Forage fibre analyses: apparatus,reagents, procedures, and some applications. Agricultural handbook 379. Washington, DC: U.S. Government Printing Office.
Ilukor, J.O. and Oluka, S.O. (1995). Carbon-tonitrogen ratios in agricultural residues. Environmental Monitoring and Assessment 38:271-275.
Islam, M.Z., Rahman, M.H. and Hafiz, F. (2009). Cultivation of oyster mushroom Pleurotus flabellatus on different substrates. International Journal of Sustainable Crop production 4: 45-48.
Jandaik, C.L. and Goyal, S.P. (1995). Farm andfarming of oyster mushroom (Pleurotus sp). In: Mushroom Production Technology (Eds. Singh, R. P. and Chaube, H. S.). G. B. Pant Univ. Agril. And Tech., Pantnagar India, pp72-78.
Jayasuriya, W.J., Suresh, T.S., Abeytunga, D., Fernando, G.H. and Wanigatunga, C.A. (2012) Oral hypoglycemic activity of culinary-medicinal mushrooms Pleurotus ostreatus and P. cystidiosus (higher basidiomycetes) in normal and alloxaninduced diabetic Wistar rats. International Journal of Medicinal Mushrooms, 14: 347– 355.
Joshua, V.I. and Agina, S.E. (2002). Cultivation of Pleurotus ostreatus on sawdust in Jos Plateau. Nigerian Journal of Botany 15: 53-56.
Krupodorova, T., Rybalko, S. and Barshteyn, V. (2014). Antiviral activity of Basidiomycete mycelia against influenza type A (serotype H1N1) and herpes simplex virus type 2 in cell culture. Virologica Sinica 29: 284–290.
Kurtzman, R.H. (1976). Nitration of Pleurotus sapidus effects of lipid. Mycologia 68: 268- 295.
Liang, Z., Wu, C., Shieh, Z. and Cheng, S. (2009). Utilization of grass plants for cultivation of Pleurotus citrinopileatus. International Biodeterioration & Biodegradation 63 (4): 509-514.
Maher, M.J. (1991). Spent mushroom compost (SMC) as a nutrient in peat based potting substrates. In Maher MJ (Ed.) Science and Cultivation of Edible Fungi. Balkema. Rotterdam, Holland. 645-650 pp.
Mamiro DP and Mamiro PS. (2011). Yield and Mushroom Size of Pleurotus ostreatus Grown on Rice Straw Basal Substrate Mixed and Supplemented with Various Crop Residues. Journal of Animal and Plant Sciences 1(10) :1211- 1218.
Meza-Menchaca, T., Suárez-Medellín, J., Del Ángel-Piña, C. and Trigos, Á. (2015). The amoebicidal effect of ergosterol peroxide isolated from Pleurotus ostreatus. Phytotherapy Research 29: 1982–1986.
Mwita, L.N., Lyantagaye, S.L. and Mshandete, A.M. (2011). The effect of the interaction ofvarying chicken manure supplement levels with three different solid sisal wastes substrates on sporocarp cap lengths and diameters, stipe lengths and diameters and dry weights of Coprinus cinereus (Schaeff) S. Gray s.lat. African Journal of Biotechnology 10 (7): 1172-1180.
Naraian, R., Sahu, R.K., Kumar, S., Garg, S.K., Singh, C.S. and Kanaujia, R.S. (2009). Influence of different nitrogen rich supplements during cultivation of Pleurotus florida on corn cob substrate. Environmentalist 29: 1-7.
Ortega, G.M., Martinez, E.O., Betancourt, D., Gonzalez, A.E. and Otero, M.A. (1992). Bioconversion of sugarcane crop residues with white rot fungi Pleurotus species. World Journal of Microbiology & Biotechnology 8(4), 402-405.
Park, C., Lim, J.S., Lee, Y., Lee, B., Kim, S.W.,Lee, J. and Kim, S. 2007). Optimization and morphology for decolorization of reactive black 5 by Funalia trogii. Enzyme and Microbial Technology 40(7):1758–1764. doi: 10.1016/j.enzmictec.2006.12.005.
Patel, S. and Goyal, A. (2012). Recent developments in mushrooms as anticancer therapeutics: a review. Biotechnology 2: 1–15.
Pathania, S., Sharma, N. and Gupta, D. A. (2017). Study on Cultivation and Yield Performance of Oyster Mushroom (Pleurotus ostreatus) on Wheat Straw Mixed with Horticultural Waste (Apple Pomace) in Different Ratio and their Nutritional Evaluation. International Journal of Current Microbiology and Applied Sciences 6(8): 2940-2953. doi: https://doi.org/10.20546/ijcmas.2017.608.353.
Philippoussis, A., Diamantopoulou, P. and Israilides, C. (2007). Productivity of agricultural residues used for the cultivation of the medicinal fungus Lentinus edodes. International Biodeterioration & Biodegradation 59 (3): 217-219.
Philippoussis A, Diamantopoulou P and Zervakis GI. (2003). Correlation of the properties of several lignocellulosic substrates to the crop performance of the shiitake mushroom Lentinus edodes. World Journal of Microbiology & Biotechnology 19(6): 551-557.
Philippoussis A and Diamantopoulou P. (2011). Agro-food industry wastes and agricultural wastes residues conversion into high value products. Proceedings of the 7th International Conference on Mushroom Biology and Mushroom Products. Archachon, France. pp. 339-351.
Pokhrel, C.P., Yadav, R.K.P and Ohga, S. (2009). Effects of physical factors and synthetic media on mycelial growth of Lyophyllum decastes. Journal of Ecobiotechnology 1: 46-50.
Purkayastha, R.P., Nayak, D. (1981). Analysis of Protein patterns of an Edible mushroom by GelElectrophoresis and its amino acid composition. Journal of Food Science and Technology 18, 89-91.
Sanches, C. (2010). Cultivation of Pleurotus ostreatus and other edible mushrooms. Applied Microbiology and Biotechnology 85: 1321–13377.
Santoyo, S., Ramírez-Anguiano, A.C., AldarsGarcía, L., Reglero, G. and Soler-Rivas, C. (2012). Antiviral activities of Boletus edulis, Pleurotus ostreatus and Lentinus edodes extracts and polysaccharide fractions against Herpes simplex virus type 1. Journal of Food and Nutrition Research 51: 225–235.
Shah, .ZA., Ashraf, M. and Ishtiaq, M.C. (2004). Comparative study and cultivation and yield performance of oyster mushroom (Pleurotus ostreatus) on different substrates (wheat straw, leaves, sawdust). Pakistan Journal of Nutrition 3 (3): 158 –160.
Sharma, S.K., Atti, N.S., Joshi, R. and Gulati, A. (2013). Evaluation of wild edible mushrooms for amino acid composition. Academic Journal of Plant Sciences 5 (2): 56-59.
Siegel, R.L., Miller, K.D., Jemal, A. (2015). Cancer statistics, CA Canadian Journal of Clinical Pharmacology 65: 5–29.
Tinoco, R., Pickard, M. and Vazquez-Duhalt, R. (2001). Kinetic differences of purified laccases from six Pleurotus ostreatus strains. Letters in Applied Microbiology 32 (5): 331-335.
Wojewoda, W. (2003). Checklist of Polish Larger Basidiomycetes. W. Szafer Institute of Botany, Polish Acad. of Sci. Kraków.
Younis, A.M., Wu, F.S. and El-Shikh, H.H. (2015). Antimicrobial activity of extracts of the oyster culinary medicinal mushroom Pleurotus ostreatus (higher basidiomycetes) and identification of a new antimicrobial compound. International Journal of Medicinal Mushrooms 17: 579–590.
Zenebe, G., Weldesemayat, G., Getachew, B. and Solomon, Z. (2016). Growth and yield performance of Pleurotus ostreatus (Jacq. P.) Kumm (Oyster mushroom) on different substrates. Current Agriculture Research Journal 6: 87-96.