Mycoflora of postharvest rots of bitter yam Dioscorea dumentorum Pax in Nsukka agro ecological zone, Nigeria.

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C. C. Onyeke
G. C. Ajuziogu
C. V. Okafor

Abstract

This study was done to recover fungi associated with postharvest rotting of bitter yam, Dioscorea dumentorum tubers in Nsukka Agroecological Zone, Nigeria. Tubers showing signs of rot were picked from farmers in four randomly selected towns in the zone. The towns included, Nsukka, Orba, Ibagwa and Obollo-Afor. Five (5) samples each were picked from three (3) randomly selected farmers. Samples were packaged in sterile polythene bags, labeled accordingly and moved to the laboratory for studies. The fungal isolates were identified using both cultural and molecular techniques. Results showed that mycoflora of rotted bitter yams in the Zone were, Aspergillus flavus, Aspergillus niger, Lasiodiplodia pseudotheobromae, Galactomyces candidum and Rhizopus delemar. The percentage frequencies of occurrence of the fungal organisms ranged from 14.03% to 26% with A. niger recording the highest (26%) while A. flavus recorded the least (14.03%). Pathogenicity tests revealed that the fungi produced rots (diameter) ranging from 1.70 cm to 2.40 cm with R. delemar recording the highest rot diameter (2.40 cm) while A. niger produced the least (1.70 cm).

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How to Cite
Onyeke, C. C., Ajuziogu, G. C., & Okafor, C. V. (2025). Mycoflora of postharvest rots of bitter yam Dioscorea dumentorum Pax in Nsukka agro ecological zone, Nigeria. Journal of Biological Research and Biotechnology, 16(1), 1017-1024. https://doi.org/10.4314/br.v16i1.3
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References

Ademoh, O.F., Afolabi, A.M., Orisasona, B.A. and Olowolaju E.D. (2017). Isolation and Identification of Rot Fungi on Post-Harvest of Pepper (Capsicum annuum L.) Fruits. AASCIT Journal of Biology 3(5) :24-29.

Anwadike, B.C. (2018). Fungal Rot of Yam (Dioscorea alata Lin.) Sold a t Nsukka Markets in Nigeria. Annual Research and Review in Biology 28 (2): 1-9.

Ayensu, E.S. and Coursey D.G. (1972). Guinea yams. Economic Botany 26:301-318.

Bai, K.V.and Ekanayake I.J. (1998). Taxonomy, morphology and floral biology. In: Orkwor, G., Asiedu, R. and Ekanayake I.J.(Eds.). Food yams:

Advances in Research, IITA/NRCRI, Nigeria Co-publication, Umudike, Nigeria. Pp: 13-37.

Bhattacharjee, R., Melaku, G., Alieu, S., Otoo, E., Dumet, D., Kikuno, H., Kumar, P.L. and Asiedu R. (2011). Dioscorea. In: Kole, C. (eds) Wild

Crop Relatives: Genomic and Breeding Resources, Industrial Crops Springer-Verlag, Berlin, Heidelberg Pp 71-76.

Bourret, T.B., Kramer, E.K., Rogers,. JD. and Glawe D.A. (2013). Isolation of Geotrichum candidum pathogenic to tomato (Solanum lycopersicum) in Washington State. North American Fungi 8(14): 1-7.

Brown, G.E. (1979). Biology and control of Geotrichum candidum, the cause of citrus sour rot. Proceedings of the Florida State Horticultural

Society. 92: 186-189.

Bryden, W.L. (2007). Mycotoxins in the food chain, human implications. Asia Pacific Journal of Clinical Nutrition. 16(1):95–101.

Burkil, H.M. (1985). The Useful Plants of West Tropical Africa. Royal Botanical Gardens. http//www.aluka.org

Cemaluk, E.A.C., Daniel, N.C.and Nkiru E.O.C. (2014). Effect of soaking prior to oven drying on some nutrients and anti-nutrient properties of bitter yam (Dioscorea dumentorum). Journal of Nutrition and Food Sciences 4: 280.

Cenis, J.L. (1992). Rapid extraction of fungal DNA for PCR amplification. Nucleic Acids Research 20:2380.

Corley, D., Tempesta, M.S. and Iwu M. (1985). Convulsant alkaloids from Dioscorea dumentorum. Tetrahedron letters 26(13): 1615-1618.

Coursey, D.G. (1967). Yam storage I. A review of storage practices and information on storage losers. Journal of Stored Product Research 2: 227-244.

de Hoog, G.S., Guarro, J., Gene, J.and Figueras M.J. (.2000). Atlas of clinical fungi 2nd edition. Centraalbureau voor Schimmelcultures Utrecht, The Netherlands/ Universitat Rovira I Virgili Reus, Spain. 1126pp.

Degras, L. (1993). The Yam, A Tropical Root Crop. Macmillan Press, London. Dissanayake, A.J., Zhang, W., Mei, L.,

Chukeatirote, E., Yan, J.Y., Li, X. and Hyde K.D. (.2015). Lasiodiplodia pseudotheobromae causes pedicel and peduncle discolouration of grapes in China Australasian. Plant Disease Notes 10:21

Ekundayo, J.A. and Naqvi, S.H.Z. (1972). Preharvest and postharvest microbial rotting of yams (Dioscorea spp.) in Nigeria. Transanctions of British Mycological Society 58(1): 15-18.

El-Shanshoury, A.R., Bazaid, S.A., El-Halmouch, Y.and Ghafar M. (2013). Control the post-harvest infection by Aspergillus spp. to Taify table grape using grape epiphytic bacteria. Life Sciences Journal 10(1):1821-1636.

Ezeh, N.O. (1998). Economics of production and postharvest Technology In: Orkwor, G.C., Asiedu, R. and Ekanayake I.J. (eds.) Food yam Advances in research IITA and NRCRI, Nigeria, pp.187-214.

Food and Agriculture Organisation(FAO). (1998). Food and Agriculture Organization of the United Nation, Yearbook 48.

Hafeez, R., Ahktar, N., Shoaib, A., Haider, M.S., Bashir.and Awan Z.A. (.2015). First report of Geotrichum candidum from Pakistan causing postharvest sour rot in Loquat (Eriobotrya japonica). The Journal of Animal and Plant Sciences 25(6): 1737-1740.

Hamon, P. and Toure, B. (1990). The classification of the cultivated yams (Dioscorea cayenensis-rotundata complex) of West Africa. Euphytica

:179–187.

Ibrahim, A., Edwards, J.E. and Filler S.G. (2004). Mucormycosis. Harcourt Brace, Philadelphia.

IITA. (2006). Yam: Research Review. International Institute of Tropical Agriculture, Ibadan, Nigeria, pp 1-4.

Lebot, V. (2008). Crop production science in horticulture. Volume 17: Tropical root and tuber crops: Cassava, sweet potato, yams and aroids.

Wallingford, Oxon: CABI Publishing.

Morse, S.M., Acholo, M.C. and Oliver R. (2000). Control of storage insect as a means limiting yam tuber fungal rots. Journal of Stored Product Research 36:37-48.

Odeku, O.A. and Picker-Freyer K.M. (2007). Analysis of the material and tablet formation properties of four Dioscorea starches. Starch 59:430–

Ogbo, C.F. and Agu C.K. (2014). Role of Fungal Rots in Post-harvest Storage Losses in Some Nigerian Varieties of Dioscorea Species. British

Microbiology Research Journal 4(3): 343-350.

Ogunleye, A.O. and Ayansola O.T.(2014). Studies of some isolated rot-causing mycoflora of yams (Dioscorea spp.). American Journal of Microbiology and Biotechnology 1(1): 9-20.

Okigbo, R.N. (2003). Fungi associated with peels of postharvest yams in storage. Global Journal of Pure and Applied Sciences 9(1): 19-23.

Okigbo, R.N. and Ikediugwu, F.E. (2000). Studies on biological control of post- harvest rot of yam (Dioscorea spp.) with Trichoderma viride. Journal of Phytopathology 148: 351–355.

Okigbo, R.N. (2005). Biodegradation of white yam (Dioscorea rotundata Poir) and bitter yam (Dioscorea alata L.) slices dried under different conditions. KMITL Science and Technology Journal 5(3):577-586.

Petrikkos, G., Skiada, A., Lortholary, O., Roilides, E., Walsh, T.J. and Kontoyiannis D.P. (2012). Epidemiology and Clinical Manifestations of

Mucormycosis. Clinical Infectious Diseases 54(S1):23–34.

Rippon, J.N. (1984). The pathogenic fungi and pathogenic actinomyces. In: Medical mycology 3rd edition. Sanders Co. Philadelphia 163pp.

Samson, R.A., Hockstra, E.S.and Van-Orschot A.N. (1984). Introduction to food borne fungi. The Netherlands Academy of Arts and Science

Sonibare, M.A., Asiedu, R. and Albach, D.C. (2010). Genetic diversity of Dioscorea dumentorum (Kunth) pax using amplified fragment length

polymorphism (AFLP) and cpDNA. Biochemical Systematics and Ecology 38: 320-334

Ukpabi, U.J. (2014). Molecular analysis of Dioscorea dumentorum in South-East Nigeria using random amplified polymorphic DNA markers.

American Journal of Microbiology and Biotechnology, 1 (2): 77-82.

Ukpabi, U.J. (2015). Traditional food processing techniques of Dioscorea dumetorum in Nigeria. American Journal of Food Science and Nutrition 2(3): 21-30.

White, T.J., Bruns, T., Lee, S. and Taylor, J. (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis, M.A, Gefland, D.H., Sninsky, J.J. and White T.J. (eds.). PCR Protocols: A Guide to Methods and Applications. Academic Press, New York; pp315-322.

Wild, C.P.and Gong Y.Y. (2010). Mycotoxins and human disease: a largely ignored global health issue. Carcinogenesis 31:71-82.

Zhao, J.P., Lu, Q., Liang, J., Decock, C. and Zhang X.Y. (2010). Lasiodiplodia pseudotheobromae, a new record of pathogenic fungus from some

subtropical and tropical trees in southern China. Cryptogamie. Mycologie 31:431–439.

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