Sublethal Effects of Organophosphate Chlorpyrifos on Hemato-Immunological Parameters of the Gercacinid Crab, Cardiosoma armatum (Herklots, 1851)
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Abstract
Low insecticide exposure has been shown to cause profound effects on non-target organisms, including crabs. Therefore, the changes in hematological parameters, serum biochemistry and antioxidant enzymes in the Gercacinid Crab, Cardiosoma armatum were assessed during 28-day exposure to four concentrations of organophosphate chlorpyrifos (0.003, 0.006, 0.03 and 0.06 mg/l). The results showed a significant (P= 0.0) decrease in packed cell volume and total haemocyte count of the exposure crabs (except in 0.003 mg/l concentration) compared to control group. There were no significant changes in hemocyte sedimentation rate, granulocyte and agranulocyte, although all exposure groups increased in hemocyte sedimentation rate and agranulocyte with respective ranges of 3.00-3.02 mm/hr and 64.00-67.00 %. Except for alkaline phosphatase, there were no significant variation in the biochemical profile of both the control crabs and exposure crabs, although organophosphate chlorpyrifos exposure induced increase in all the measured biochemical
parameters. The serum protein level and the activities of the enzymes (superoxide dismutase, catalase and malondialdehyde) were inhibited in exposure groups. The changes in these hemato-immunological parameters of the crabs were suitable biomarkers of a sub-lethal exposure to chlorpyrifos at the concentrations tested, and this will be useful in biomonitoring of aquatic environment.
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References
Adewumi, B., Ogunwole, G. A., Akingunsola, E., Falope, O. C. and Eniade, A. (2018). Effects of sub-lethal toxicity
of chlorpyrifos and DDforce pesticides on haematological parameters of Clarias gariepinus. International Journal of Environmental Research and Public Health.,5(5):62-71.
Aliko, V., Hajdaraj, G., Caci, A. and Faggio, C. (2015). Copper induced lysosomal membrane destabilisation in
hemolymph cells of Mediterranean green crab (Carcinus aestuarii, Nardo, 1847) from the Narta Lagoon (Albania). Brazilian Archives of Biology and Technology., 58(5):750–756.
Anguirre-Martinez, G. V., Burrati, S., Fabri, E., Del Valls, T. A. and Martin-Diaz, M. L. (2013). Stability of lysosomal membrane in Carcinus maenas acts as biomarker of exposure to pharmaceuticals. Environmental Monitoring and. Assessment., 185 (5):3783–3793.
Bertholdo-Vargas, L. R., Martins, J. N., Bordin, D., Salvador, M., Schafer, A. L., Barros, N. M., Barbieri, L., Stirpe, F. and Carlini, C. R. (2009). Type 1 ribosome-inactivating proteins- entomotoxic, oxidative and genotoxic action on Anticarsia gemmatalis (Hubner) and Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae). Journal of Insect Physiology, 55(1): 51-58.
Blaxhall, P. C. and Daisley, K. W. (1973). Routine haematological methods for use with fish blood. Journal of Fish Biology, 5: 771-781.
Bueno-Krawczyk, A. C. D., Guiloski, I. C., Piancini, L. D. S., Azevedo, J. C., Ramsdorf, W. A., Ide, A. H. and De Assis, H. S.
. Multi-biomarker in fish to evaluate a river used to water public supply. Chemosphere, 135: 257-264.
Coles, E. H. (1986). Veterinary Clinical Pathology. W.B. Saunders, Philadelphia, PA, USA, 1-42.
Duarte-Restrepo, E., Jaramillo-Colorado, B. E.and Duarte-Jaramillo, L. (2020). Effects of chlorpyrifos on the crustacean Litopenaeus vannamei. PloS One, 15(4), e0231310.
Gomes, T., Pereira, C. G., Cardoso, C., Pinheiro, J. P., Cancio, I. and Bebianno, M. J. (2012). Accumulation and toxicity of copper oxide nanoparticles in the digestive gland of Mytilus galloprovincialis. Aquatic Toxicology, 118: 72-79.
Ighodaro, O. M. and Akinloye, O. A. (2018). First line defence antioxidants- superoxide dismutase(SOD), catalase (CAT) and glutathione peroxidase (GPX): Their fundamental role in the entire antioxidant defence grid. Alexandria Journal of Medicine, 54(4): 287-293.
Jacobo, L.L., Diaz, F., Re, A.D., Galindo- Sanchez, C.E., Sanchez-Lizarraga, A.L., Nunez- Moreno, L.A. and Moreno-Sierra,
D. (2016). Physiological responses of the red rocky crab, Cancer antennarius,exposed to different concentrations of copper sulphate. Revista de Biologia Marina y Oceanografia, 51 (2): 327–336.
Lawal-Are, A. O., Moruf, R. O., Oluseye-Are, S.O. and Isola, T. O. (2019a). Antioxidant defense system alternations in four crab species as a bio-indicator of environmental contamination. Bulletin of University of Agricultural Sciences and
Veterinary Medicine., 76(1): 73-80.
Lawal-Are, A. O., Moruf, R. O., Akubueze, K. L. and Adewole, O. (2019b). Morphometric characteristics of two walking crabs, Cardiosoma armatum (Gecarcinidae) and Goniopsis pelii (Graspidae) from a tropical mangrove swamp in Lagos, Nigeria. Nigerian Journal of Agriculture, Food and Environment, 15(1): 83-88.
Lushchak, V. I., Bagnyukova, T. V., Huska, V. V., Luzhna, L. I., Lushchak, O.V. and Storey, K. B. (2005). Hyperoxia results in transient oxidative stress and an adaptive response by antioxidant enzymes in gold tissues. International Journal of Biochemistry and Cell biology, 37(8): 1670-1680.
Moruf, R. O. and Akinjogunla, V. F. (2019). Concentration of heavy metals in sediment of two interconnecting brackish/freshwater lagoons and the bioaccumulation in the crustacean, Farfantepenaeus notialis (Pérez- Farfante, 1967). Journal of Fisheries and Environment, 43 (3): 55-62.
Moruf, R. O. and Lawal-Are, A. O. (2015). Growth Pattern, Whorl and Girth Relationship of the Periwinkle, Tympanotonus fuscatus var radula (Linnaeus, 1758) from a Tropical Estuarine Lagoon, Lagos, Nigeria. International Journal of Fisheries
and Aquatic Studies, 3 (1):111-115.
Moruf, R. O. and Lawal-Are, A. O. (2018). Haemato-biochemical variations in estuarine crabs from a lagoon ecosystem. Journal of Applied Sciences and Environmental Management, 22(12): 1899–19033.
Paracampo, A., Solis, M., Bonetto, C. and Mugni, H. (2015). Acute toxicity of chlorpyrifos to the non-target organism
Cnesterodon decemmaculatus. International Journal of Environmental Health Research, 25(1): 96-103.
Qyli, M. and Aliko, V. (2016). The toxic effects of chloroform exposure on the Mediterranean green crab, Carcinus aestuarii. Albanian Journal of Agricultural Sciences, 15 (4): 177– 182.
Rasheed, M. A., Khan, M. M., Hafeez, M., Zhao, J., Islam, Y., Ali, S., ... & Zhou, X. (2020). Lethal and Sublethal effects of chlorpyrifos on biological traits and feeding of the aphidophagous predator Harmonia axyridis. Insects., 11(8): 491-506.
doi:10.3390/insects11080491
Sanni, Z. A., Moruf, R. O. and Lawal-Are, A. O. (2020).Hemato-biochemical profiling of a burrowing crab exposed to
polystyrene microplastic contaminant. Federal University of Dutsin-Ma Journal of Science., 4(2): 380-385.
Singaram, G., Harikrishnan, T., Chen, F. Y., Bo, J. and Giesy, J. P. (2013). Modulation of immune-associated parameters
and antioxidant responses in the crab (Scylla serrata) exposed to mercury. Chemosphere, 90(3): 917-928.
Sussarellu, R., Fabioux, C., Sanchez, M. C.,Le Goïc, N., Lambert, C., Soudant, P. and Moraga, D. (2012). Molecular and cellular response to short-term oxygen variations in the Pacific oyster Crassostrea gigas. Journal of Experimental Marine Biology and Ecology, 412: 87-95.
Usese, A. I., Elike, M. I., Moruf, R. O. and Chukwu, L. O. (2019). Levels of oxidative stress markers in the Mangrove
Oyster, Crassostrea gasar from a coastal ecosystem in Southwest Nigeria. Journal of Research in Forestry, Wildlife &
Environment, 11(1): 32 – 38.
Velmurugan, B., Satar, E. I., Yolcu, M., Uysa, E., Selvanayagam, M. and Senthilkumaar, P. (2019). Sublethal effects of chlorpyriphos on some hematological parameters in freshwater fish Anabas testudineus. Acta Biologica Turcica, 32(4): 173- 180.