Evaluation of bioactiveconstituents and In vitro antioxidant potentials of the ethanolic leaf extracts of Dracaena mannii, Euphorbia hirta and Senna alata
Article Sidebar
Main Article Content
Abstract
The current study investigated the phytochemical constituents and in vitro antioxidant activities of the ethanolic leaf extracts of Dracaena mannii, Euphorbita hirta and Senna alata. The phytochemical analyses of the leaf extracts showed varying amounts of important compounds such as tannins, phenols, alkaloids, flavoniods, protein and carbohydrates. Findings from the in-vitro free radical mopping abilities of the ethanolic leaf extracts against 1,1-diphenyl-2-picryl hydrazyl and nitric oxide radical (NO•) as well as the reducing power and total antioxidant capacities revealed abilities to quell oxidative stress with S. alata > E. hirta > D. mannii excluding the NO• radical inhibition activity. This relationship was also reflected in the IC50/EC50 values obtained, suggesting that the S. alata ethanolic leaf extract has the best antioxidant potential while E. hirta showed better activities than D. mannii. Thus, the leaves of S. alata could be further investigated for other biological potentials and possibly employed in the application of
leaves against diseases that are implicated by oxidative stress.
Downloads
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
References
Adedayo, O., Anderson, W.A., Moo-Young, M., Snieckus, V., Patil, P.A. and Kolawole, D.O. (2001). Phytochemistry and antibacterial activity of Senna alata flower. Pharmaceutical Biology, 39(6): 408-412.
Adjeroh, L.A., Ajuruchi, V.C., Nnokwe, J.C., Azuwike, C.O. and Mgbemena, I.C. (2015) Preliminary phytochemical screening and antimicrobial/antifungal activities of leaf extracts of Euphorbia hirta. Journal of Natural Sciences Research, 5 (16): 79-84.
Al-Snafi, A.E. (2017). Pharmacology and therapeutic potential of Euphorbia hirta (Syn: Euphorbia pilulifera) - A Review. IOSR Journal of Pharmacy, 7(3): 7-20.
Anigboro, A.A., Onakurhefe, P., Tonukari, N.J., Avwioroko, O.J. and Egbeme, E. (2014). Quantitative determination of some phytochemicals (phenol, flavonoid, saponin and alkaloid) in twenty-two Nigerian medicinal plants. Nigerian Journal of Science and Environment, 13(1): 86-93.
Anigboro, A.A, Avwioroko, O.J., Ohwokevwo,O.A. and Pessu, B. (2019). Bioactive components of Ficus exasperata, Moringa oleifera and Jatropha tajorensis leaf extracts and their evaluation of their antioxidant
properties. Eurasian Journal of BioSciences, 13: 1763-1769.
Anigboro, A.A. (2017). Protective effects of Luffa aegyptica aqueous extract against biochemical alterations in diabetic rats. NISEB Journal, 17(3): 102 – 110.
Anigboro, A.A. (2018). Antimalarial efficacy and chemopreventive capacity of bamboo leaf (Bambusa vulgaris) in malaria parasitized mice. Journal of Applied Science and Environmental Management, 22(7): 1141–1145.
Anigboro, A.A., Avwioroko, O.J. and Cholu, C.O. (2020). Phytochemical constituents, antimalarial efficacy, and protective effect of Eucalyptus camaldulensis aqueous leaf extract in plasmodium berghei-infected mice. Preventive Nutrition and Food Science,25(1): 58-64.
Anigboro, A.A., Avwioroko, O.J., Akeghware, O. and Tonukari, N.J. (2021). Antiobesity, antioxidant and in silico evaluation of Justicia carnea bioactive compounds as potential inhibitors of an enzyme linked with obesity: Insights from kinetics, semi-empirical quantum mechanics and molecular docking analysis. Biophysical Chemistry, 274:106607.
Anigboro, A.A., Avwioroko, O.J., Ohwokevwo, O.A. and Nzor, J.N. (2018). Phytochemical constituents,
antidiabetic and ameliorative effects of polyalthia longifiola leaf extract in alloxan-induced diabetic rats. Journal of Applied Science and Environmental Management, 22 (6): 993 –998.
Anigboro, A.A., Avwioroko, O.J., Tonukari, N.J. (2018). Brillantasia patula aqueous leaf extract averts hyperglycermia, lipid peroxidation, and alterations in hematological parameters in alloxaninduced diabetic rats. International Journal of Biomedical Science and Engineering, 6(2): 43-51.
Borokini, T.I. and Omotayo, F.O. (2012). Comparative phytochemical analysis of selected medicinal plants in Nigeria. International Journal of Advanced Chemical Research, 1(1): 11-18.
Damen, T.H.J., Vander-Burg, W.J., WilandSzymańska, J. and Sosef, M.S.M. (2018). Taxonomic novelties in African Dracaena (Dracaenaceae). BlumeaBiodiversity, Evolution and Biogeography of Plants, 63(1): 31-53.
Dzialo, M., Mierziak, J., Korzun, U., Preisner, M., Szopa, J. and Kulma, A. (2016). The potential of plant phenolics in prevention and therapy of skin disorders. International Journal Molecular Science, 17(2): 160-201.
Essiett, U.A. and Okoko, A.I. (2013). Comparative nutritional and phytochemical screening of the leaves
and stems of Acalypha fimbricata Schum. & Thorn. and Euphorbia hirta Linn. Bulletin of Environment,
Pharmacology and Life Sciences, 2(4):38-44.
Ghosh, P., Chatterjee, S., Das, P., Karmakar, S. and Mahapatra, S. (2019). Natural habitat, phytochemistry and pharmacological properties of a medicinal weed – Cleome Rutidosperma DC. (Cleomaceae): A
comprehensive review. International Journal of Pharmaceutical Sciences and Research, 10(4): 1000-1008.
Gornall, A., Bardsmill, C.T. and David, M.M. (1949). Determination of serum proteins by means of the Biuret
reaction. The Journal of Biological Chemistry, 177: 751-766.
Ilodibia, C.V., Ugwu, R.U., Okeke, C.U., Akachukwu, E.E., and Ezeabara, C.A. (2015). Phytochemical evaluation of various parts of Dracaena arborea Link and Dracaena mannii Bak. African Journal of Plant Science, 9(7): 287-292.
Jia, Z., Tang, M. and Wu, J. (1999). The determination of flavonoid contents of murlberry and their scavenging effects on superoxide radicals. Food Chemistry, 64: 555-559.
Mahmoudi, S., Khali, M., Benkhaled, A., Benamirouche, K. and Baiti, I.(2016). Phenolic and flavonoid contents, antioxidant and antimicrobial activities of leaf extracts from ten Algerian Ficuscarica L. varieties. Asian PacificJournal Tropical. Biomedical, 6: 239–245.
Manzocco, L., Anese, M. and Nicoli, M.C. (1998). Antioxidant properties of tea extracts as affected by processing. Lebens-mittel-Wissenschaft UndTechnologie, 31(7–8): 694–698.
Marcocci, I., Marguire, J.J., Droy-lefaiz, M.T. and Packer, L. (1994). The nitric oxide scavenging properties of Ginkgo biloba extract. Biochemical Biophysical Research Communications, 201: 748–755.
Miller, G.L. (1959). Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical Chemistry, 31(3):426-428.
Mohammad, A.B.R., Zakaria, Z., Sreenivasan, S., Lachimanan, Y.L. and Santhanam, A. (2010). Assessment of Euphorbia hirta L. leaf, flower, stem and root extracts for their antibacterial and antifungal activity and brine shrimp lethality. Molecules, 15: 6008-6018.
Mutiu, I. K., Ganiyat, A. A., Anofi, O. T. (2015). Effect of Senna alata (L) Roxb (L) Roxb (Fabaceae) leaf extracts on alphaamylase, alpha-glucosidase and postprandial hyperglycemia in rats. Tropical Journal of Pharmaceutical Research, 14(10): 1843-1848.
Njoku, O.V. and Chidi, O. (2009). Phytochemical constituents of some selected medicinal plants. African
Journal of Pure and Applied Chemistry, 3(11): 228-233.
Nyamai, D.W., Arika, W., Ogola, P.E., Njagi, E.N.M. and Ngugi, M.P. (2016). Medicinally important phytochemicals: An untapped research avenue. Journal of Pharmacognosy and Phytochemistry, 4(1): 35-49.
Oyaizu, M. (1986). Studies on products of browning reactions: antioxidant activities of products of browning reaction prepared from glucosamine. Journal of Nutrition, 44: 307-315.
Prieto, P., Pineda, M. and Aguilar, M. (1999). Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Analytical Biochemistry, 269(2): 337-341.
Shamsa, F., Monsef, H., Ghamooshi, R. and Verdain-rizi (2008). Spectrophotometric determination of
total alkaloids in some Iranian medicinal plants. Thai Journal of Pharmaceutical Science, 32: 17-20.
Sharma, N.K. and Prasad, R. (2008). Oxidative injury to protein and their protection by phenolic acid anti-oxidants from Euphorbia hirta leaves Abstracts. Journal of Biotechnology, 136: 717-742.
Singleton, V.L. and Rossi, J.A. (1965). Colorimetry of total phenolics with phosphomolybdic-hosphotungstic
acid reagents. American Journal of Enology and Viticulture, 16: 144-158.
Thu, Z.M., Myo, K.K., Aung, H.T., Armijos, C. and Vidari, G. (2020). Flavonoids and Stilbenoids of the Genera Dracaena and Sansevieria: Structures and Bioactivities. Molecules, 25(11): 2608-2613.
Wilcox, J.R., Premachandra, G.S.. Young, K.A. and Raboy, V. (2000). Isolation of high seed inorganic P, low-phytate soybean mutants. Crop Science, 40:1601-1605.