Glutathione and Lipid Peroxidation Profiles of Normal, G6PD-Deficient and Sickle Erythrocytes Exposed to Oxidative Stress
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Abstract
In this study, the glutathione (GSH) and lipid peroxidation profiles of normal, G6PD-deficient and sickle erythrocytes were assessed before (pre-APHZ) and after (Post-APHZ) induction of oxidative stress with acetyphenylhydraizine (APHZ). Lipid peroxidation was measured as the amount of malondialdehyde (MDA) produced per unit time (nmol hr 1 ). Before treating the erythrocytes with APHZ, the mean GSH levels were 39.82 ± 1.48, 36.00 ± 2.44 and 26.00 ± 2.32 mg /10 ml in normal subjects, G6PD-deficient subjects and sickle
cell patients respectively. The post-APHZ treatment levels of GSH for the same categories of erythrocytes were 37.0 ± 3.25, 17.10 ± 0.011 and 13.00 ± 1.98 mg/ 100 ml respectively. This study revealed that in the absence of an oxidant stressor, normal red cells, and those of G6PD-deficient subjects maintain high levels of GSH and low levels of lipid peroxidation products (measured as malondialdehyde, MDA) and that the initial effects of exposure to oxidative stress are lower GSH and elevated MDA contents of the cells. This study further established that the erythrocytes of sickle cells patients, even without any exogenous oxidative challenge, have subsisting low levels of GSH and high levels of MDA, most probably because sickle cell complications give rise to high levels of redox species that constitute molecular threats to the integrity of red blood cells. It was also a finding in this study that the red cells of normal subjects respond less significantly to such oxidative stress-induced parameters of assessing red blood cell integrity-GSH content and lipid perodixation.
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References
Bagchi, K. and Puri, S. (1998). Free radicals and antioxidants in health and disease. Eastern Mediterranean Health Journal 899: 375-391.
Baumber, B.A., Ball, C. G., Gravance, V. M. and Davies-Morel, M. C. (2000). The effect of reactive oxygen species on equine sperm motility, viability, acrosomal integrity, mitochondrial membrane potential, and membrane lipid peroxidation. Journal of Andrology, 21 (6): 895-902.
Beutler, E. (1966). A series of new screening procedures for pyruvate kinase, glucose-6-phosphate, and glutathione. Blood, 28: 553-562.
Bilgin-Kavabulut, A., Ademoglu, E., Aydin, I., Erer, M. and Gokkusu, C. (2001). Protective effects of vitamins A and E pretreatment in venous ischemia/perfusion injury. Journal of Reconstructive and Microsurgery, 17 (6): 425-429.
Cooper, C. E., Vollacird, N. B., Choueriri, T. and Wilson, M. T. (2002). Exercise, free radicals and oxidative stress. Biochemistry Society Transactions, 30 (2): 280-285.
Eze, M. O. (1991). Production of Superoxide by microphage from Plasmodium Chalamdi- infected mice. Cytobios, 93: 98-104.
Ho, J.C., Chang-Yeung, M., Ho, S. P., Mak, J.C., IP, M.S., Ooi, G. C., Wong, M. P., K. W. and Lam, W. K. (2007). Disturbance of systemic antioxidant profile in non-small lung carcinoma. European Respiration Journal, 29: 273-278.
Siroev, E. A. and Makarova, V. G. (1989). Laboratory Manual Biochemistry. Mir Publishers. Moscow.
Ho, J. C., Mark, J. C. W., Ho, S. P. IP, M. S. M. Tsang, K. W., Law, W. K and Chang-Yeung, M. (2006). Manganese Superoxide dismultage and catalase genetic polymorphisms, activity levels, and lung cancer risk in Chinese Hong Kong. Journal of Therapeutic Oncology, 1 (7): 648-653.
Snyder, R. O. V., Parke, J. J., Kocsis, D. J., Jollow, G. G. G and Witmer, C. M. (1982). Biological Reactive Intermediates. Plenum Press. New York. 783-866.
Uday, B., Dipak, D and Banerjee, R. K. (1999). Reactive oxygen species: Oxidative damage and pathogenesis (A Review). Current Science, 77: 658-663.
Johnson, C. (2000). Acute admissions of patients with sickle cell disease who live in Britain. British Medical Journal, 300: 1206-1208.
Langseth, L. (1996) Oxidants, antioxidants and disease prevention. 1 st edition: International Life Science Institute Belgium.
Mason, R. P and Chignell, C. F. (1982). Organic and Inorganic Free Radicals. Pharmacology Review, 33: 189-205.
Siroev, E. A. and Makarova, V. G. (1989). Laboratory Manual Biochemistry. Mir Publishers. Moscow.
Snyder, R. O. V., Parke, J. J., Kocsis, D. J., Jollow, G. G. G and Witmer, C. M. (1982). Biological Reactive Intermediates. Plenum Press. New York. 783-866.
Uday, B., Dipak, D and Banerjee, R. K. (1999). Reactive oxygen species: Oxidative damage and pathogenesis (A Review). Current Science, 77: 658-663.
Voet D and Voet, J. G. (1994). Biochemistry. Second Edition. John Willey and Sons. New York