Repellence of Aedes aegypti with oils from Citrus sinensis and Citrus paradisi fruit peelsi from Nsukka
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Abstract
Aedes aegypti spread yellow fever, dengue fever, Zika virus and Chikungunya that can be prevented through
vector control. Chemical insecticides are resisted by vectors, harm humans and the ecosystem thereby
making their use opprobrious. Plants essential oils are safer alternatives. Oils of Citrus sinensis and Citrus
paradisi fruit peels are readily available in Nsukka but their effect on Aedes aegypti remain uncertain so it
became imperative to verify the effect of these oil extracts on them. The cold maceration method was used
to extract essential oils from their peels. They were used singly and in various formulations to evaluate the
behaviour of starved Ae. aegypti adults. WHO protocols were applied in obtaining the effective doses and
complete protection time. Data collected were analysed using SPSS version 23.0. Various formulations
repelled mosquitoes in the magnitude of 100 % > 75 % > 50% > 25% and significant differences (p < 0.05)
noted between formulations. Formulations B and H showed highest repellence effects with 0 – 3 number of
mosquito landings during the three minutes of test period. Probit analysis showed that 1.14 ml of formulation
G was required to achieve 99% repellence. Formulation B demonstrated synergism of the oils attaining the
highest complete protection time of 150 minutes. This study showed that oil extracts of C. sinensis and C.
paradisi would prevent female Aedes aegypti adults from landing on or feeding from skins smeared with each
of the oil or their combinations. The oils exhibited synergism when combined hence could be used to control
this mosquito.
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References
Akarca, G., and Sevik, R. (2021). Biological Activities of Citrus limon L. and Citrus sinensis L. Peel essential oils. Journal of Essential Oil Bearing Plants, 24(6): 1415-1427.
Akhtar, N., Idrees, M., ur Rehman, F., Ilyas, M., Abbas, Q., and Luqman, M. (2021). Shape and texture-based classification of Citrus using principal component analysis. International Journal of Agricultural Extension, 9(2): 229-238.
Belenje, A., Bose, R., and Jalali, S. (2021). Rare case of Terson’s syndrome and viral retinitis due to dengue haemorrhagic fever in an infant. British Medical Journal Case Reports, 14(5): e242274.
Bessimbaye, N., Mbanga, D., Moussa, A. M., Ferdinand, D. Y., Maxime, N. E. D., Barro, N., Tidjani, A and Ouchemi, C. (2021). Evaluation of yellow fever surveillance in Chad, 2015-2020. African Journal of Microbiology Research, 15(3): 152-160.
Canali, M., Rivas-Morales, S., Beutels, P., and Venturelli, C. (2017). The cost of Arbovirus disease prevention in Europe: area-wide integrated control of tiger mosquito, Aedes albopictus, in EmiliaRomagna, Northern Italy. International Journal of Environmental Research and Public Health, 14(4): 444.
Chen, G., Zhang, B., and Zhao, J. (2015). Dispersion process and effect of oleic acid on properties of cellulose sulfateoleic acid composite film. Materials, 8(5):2346-2360.
Dickens, B. L., Sun, H., Jit, M., Cook, A. R., and Carrasco, L. R. (2018). Determining environmental and anthropogenic factors which explain the global distribution of Aedes aegypti and Ae. albopictus. British Medical Journal Global Health, 3(4): e000801.
Elsey, H., Agyepong, I., Huque, R., Quayyem, Z., Baral, S., Ebenso, B., Kharel, C.,Shawon,R.A., Onwujekwe, O.,Uzochukwu,B., Nonvignon,
J.,Aryeteey, J.C., Kane,S.,Ensor,T and Mirzoev, T. (2019). Rethinking health systems in the context of urbanisation: challenges from four rapidly urbanising low-income and middle-income countries. British Medical Journal Global Health, 4(3): e001501.
Essombe Malolo, F. A., Kouam, A. D. K., Nyobe, J. C. N., Ngah, L., Frese, M., Ndom, J. C., Langat., M.K., Lenta, B.N.,Mulholland,D.A.,Sewald,N. and Wansi, J. D. (2023). Coumarinolignoid and indole alkaloids from the roots of the hybrid plant Citrus paradisi Macfad (Rutaceae). Molecules, 28(3): 1078.
Heidari, F., Sarailoo, M., Ghasemi, V., and Nadimi, A. (2017). Toxic and oviposition deterrence activities of essential oils from Citrus sinensis (L.) Osbeck and Citrus paradisi (Macfarlane) fruit peel against adults of Tribolium castaneum (Herbst). Journal of Crop Protection, 6(1): 79-88.
Favela-Hernández, J. M. J., González-Santiago, O., Ramírez-Cabrera, M. A., EsquivelFerriño, P. C., and Camacho-Corona, M. D. R. (2016). Chemistry and Pharmacology of Citrus sinensis. Molecules, 21(2): 247.
Finney, D. J. (1952). Probit analysis: a statistical treatment of the sigmoid response curve. Cambridge University Press, Cambridge.
Gillij, Y. G., Gleiser, R. M. and Zygadlo, J. A. (2008). Mosquito repellent activity of essential oils of aromatic plants growing in Argentina. Bioresource Technology, 99(7): 2507 – 2515.
Gomes, P. R. B., de Sousa, D. A., Everton, G. O., Fontenele, M. A., e Marques, J. N. L., de Freitas, A. C., ... and Mouchrek Filho, V. E. (2020). Chemical composition and larvicidal activity of Citrus limonia Osbeck bark essential oil. Journal of Medicinal Plants Research, 14(7): 354-358.
Hussain, T., Rashid, Z., Kumar, J., and Kumar, D. (2022). Rare case of dengue encephalitis with extensive brain lesions from Pakistan. BMJ Case Reports CP, 15(11):e250271.
Ibtehal, K. S. and Sarah, J. S. (2015). Extraction of essential oil from citrus by-products using microwave steam distillation. Iragi Journal of Chemical and Petroleum Engineering, 16(3): 11 – 22.
Ivoke, N., Ogbonna, P. C., Ekeh, F. N., Ezenwaji, N. E., Atama, C. I., Ejere, V. C., Onoja, S. U. and Eyo, J. E. (2013). Effects of grapefruit (Citrus paradisi MACF) (Rutaceae) peel oil against developmental stages of Aedes aegypti(Diptera: Culicidae). Southeast Asian Journal of Tropical Medicine and Public Health, 44(6): 970 – 978.
Kamal, G. M., Anwar, F., Hussain, A. I., Sarri, N. and Ashraf, M. Y. (2011). Yield and chemical composition of citrus essential oil as affected by drying pre-treatment of peels. International Food Research Journal, 18(4): 1275 – 1282.
Karunamoorthi, K., and Hailu, T. (2014). Insect repellent plants traditional usage practices in the Ethiopian malaria epidemic-prone setting: an ethnobotanical survey. Journal of Ethnobiology and Ethnomedicine, 10: 1-12.
Khanikor, B., Adhikari, K., and Rabha, B. (2021). Citrus essential oils: a suite of insecticidal compounds. Eds: Muhammad Sarwar Khan and Iqrar Ahmad Khan. Citrus–Research, Development and Biotechnology, IntechOpen: DOI: 10.5772/intechopen.95887
Kim, S. I., Yoon, J. S., Jung, J.W., Hong, K. B., Ahn, Y. J., and Kwon, H.W. (2010).Toxicity and repellency of origanum essential oil and its components against Tribolium castaneum (Coleoptera: Tenebrionidae) adults. Journal of AsiaPacific Entomology, 13: 369 – 373.
Marques, D. M., de Freitas Rocha, J., de Almeida, T. S., and Mota, E. F. (2021). Essential oils of caatinga plants with deletary action for Aedes aegypti: A Review. South African Journal of Botany, 143: 69-78.
Odo, G. E., Agwu, E. J., and Haruna, A. S. (2015). Culicid forms distribution and breeding sites in Nsukka ecological zone of South Eastern Nigeria. Journal of Parasitology and Vector Biology, 7(5): 94-100.
Othman, S. A., and Fadzil, N. F. (2021). Utilization and valorization of Citrus Fruit by-products: A Review. Enhanced Knowledge in Sciences and Technology, 1(2): 170-176.
Paixão, E. S., Teixeira, M. G., and Rodrigues, L. C. (2018). Zika, chikungunya and dengue: the causes and threats of new and re-emerging arboviral diseases. British Medical Journal Global Health, 3(1): e000530.
Paixão, E. S., Fernandes, Q. H. R. F., Cardim, L. L., Pescarini, J. M., Costa, M. C. N., Falcão, I. R.,Brickley,E.B.,Santos, A.C.,Souza, A.P.,CarvalhoSauer,R.C.O.,Smeeth,L.,Rodrigues, L.C.,Barreto, M.L and Teixeira, M. G. (2022). Socioeconomic risk markers of congenital Zika syndrome: a nationwide, registry-based study in Brazil. British Medical Journal Global Health, 7(9):e009600.
Paoli, M., de Rocca Serra, D., Tomi, F., Luro, F., and Bighelli, A. (2016). Chemical composition of the leaf essential oil of grapefruits (Citrus paradisi Macf.) in relation with the genetic origin. Journal of Essential oil Reearch, 28(4): 265-271.
Prabasari, I. (2014). Peel Ultrastructure During Developmental Stages of Citrus Sinensis(L.) Osbeck. Planta Tropika: Jurnal
Agrosains (Journal of Agro Science), 2(1): 1-11.
Rajani, M., Shrivastava, N., and Ravishankara, M. N. (2000). A rapid method for isolation of andrographolide from Andrographis paniculata Nees (Kalmegh). Pharmaceutical Biology, 38(3): 204 –209.
Soares, I. M. N., Polonio, J. C., Zequi, J. A. C., and Golias, H. C. (2022). Molecular techniques for the taxonomy of Aedes Meigen, 1818 (Culicidae: Aedini): a review of studies from 2010 to 2021. Acta Tropica: 106694.
Simon-Oke, I. A. and Akeju, A. V. (2019). Laboratory evaluation of extract from peels and seeds of some Citrus species against Anopheles mosquitoes (Diptera: Culicidae). Journal of Mosquito Research, 9(1): 1 – 8.
Singh, J., Sharma, V., Pandey, K., Ahmed, S., Kaur, M. and Sidhu, G. S. (2021). Horticultural classification of Citrus cultivars. Citrus Research, Development and Biotechnology: 1-24.
Soni, M., Bhattacharjee, C. K., Khan, S. A., and Dutta, P. (2018). DNA barcoding as a complementary approach for species identification from dengue endemic regions of North East India. International Journal of Mosquito Research, 5: 46-52.
Srivastava, A., and Kachhwaha, N. (2019). A review: Recent outbreak of Zika viruses in India, its vectorology and virology. Research & Reviews: A Journal of Microbiology and Virology, 9(2): 25-33.
Thompson, R., Martin Del Campo, J., and Constenla, D. (2020). A review of the economic evidence of Aedes-borne arboviruses and Aedes-borne arboviral disease prevention and control strategies. Expert review of vaccines, 19(2): 143-162.
Ugwu, F. S. O. and Onwuzurike, J. C. (2018). Palm wines as potent attractant to mosquitoes. Animal Research International, 15(3): 3055 – 3064.
Ugwu, F. S. O. and Oyeagu, U. (2023). Mediators of arrested development as attractive malaria vector control tools: The Azadirachta indica and azadirachtin routes. Journal of Biological Research & Biotechnology, 21(1): 1805-1817.
World Health Organisation (2009). Guidelines for efficacy testing of mosquito repellents for human skins. WHO, Geneva, WHO/HTM/NTD/WHOPES/2009.4.
Wu, G. A., Terol, J., Ibanez, V., López-García, A., Pérez-Román, E., Borredá, C. and Curk, F. (2018). Genomics of the origin and evolution of Citrus. Nature, 554 (7692): 311-316.