Partial Purification and Characterisation of Carboxymethyl Cellulase from Bacillus sp. Isolated from Sugar Cane Baggase (SCB) Dump Site
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Abstract
Carboxymethylcellulase (CMCase) was extracted from Bacillus sp. isolated from sugar cane baggase dump site. The enzyme was partially purified using ammonium sulphate precipitation and Sephadex G-200 gel and characterized at various pH and temperature values. The optimum pH of the purified enzyme was pH 6.5 and its temperature optimum was 50oC. The Michaelis-Menten constants, Km and Vmax, of the enzyme using carboxy-methyl cellulose as substrate were found to be 5 and 0.89 μmol/min respectively.
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