Evaluation of the In-Vitro Combined Antimicrobial Activities of Garcinia kola Heckel and Honey
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Abstract
The antibacterial activities of Garcinia kola and honey were evaluated against pure cultures of Staphylococcus aureus, Bacillus subtilis and Pseudomonas aeruginosa using the agar diffusion method. The combined antibacterial effects of various combination ratios of Garcinia kola extract and honey were further evaluated using a modified checkerboard method. Results show that Garcinia kola had minimum inhibitory concentration values of 0.25 mg/ml, 0.06 mg/ml and 0.25 mg/ml against S. aureus, B. subtilis and P. aeruginosa respectively while honey had MIC values of 1.8 mg/ml, 1.8 mg/ml and 0.45 mg/ml respectively. The results also showed higher sensitivity of the test isolates to a combination of Garcinia kola and honey than the individual agents. These results, therefore, indicate that combination of the Garcinia kola extract and honey could have some superior therapeutic benefits over the materials used singly.
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References
Abonyi, D.O. (2000). In vitro antiviral effects of extracts from the Lichen Parmelina perlata (L) and Garcinia kola Heckel on some selected viruses. M.Sc Dissertation. Department of Pharmaceutics pp 22
Bose, B. (1982). Honey or sugar in treatment of infected wounds? Lancet 1(8278): 963.
Brady N. F., Molan P. C. and Harfoot C. G. (1997). The sensitivity of dermatophytes to the antimicrobial activity of manuka honey and other honey. Pharm Sci., 2: 1-3.
Condon, R.E. (1993). Curious interaction of bugs and bees. Surgery, 113(2): 234 - 235.
Green A. E. (1988). Wound healing properties of honey. Br J Surg., 75(12): 1278.
Hussain, R.A., Owegby, A.G., Parimoo, P. and Waterman, P.G. (1982). Kolanone, a nobel polyisoprenylated benzophenone with antimicrobial properties from the fruit of Garcinia kola. Planta Medica, 44:78-81
Hutton, DJ. (1966). Treatment of pressure sores. Nurs Times 62(46): 1533 - 1534.
Iwu, M.M. (1984). Biflavonoids and Glycosides of Garcinia kola. Planta Medica, 45:146
Iwu, M.M. (1985) Garcinia kola: Biflavonoids and forms for the treatment of hepatitis and hepatoxicity. Nigerian Patent, 426: 82
Iwu, M.M. (1993). Handbook on African Medicinal plants. CRC Press, Boca Raton, Florida.
Keast-Butler J. (1980). Honey for necrotic malignant breast ulcers. Lancet; 2(8198): 809.
Levan, M., Vanden-Berghe, D.A., Mertens, F., Vlietinck, A. and Lammens, E. (1979). Screening of higher plants for biological activities 1. Antimicrobial activity. Planta medica, 36: 311-321
Molan P. C. (1992). The antibacterial activity of honey. 1. The nature of the antibacterial activity. Bee World, 73(1): 5-28.
Mossel, D. A. (1980). Honey for necrotic breast ulcers. Lancet 2(8203): 1091.
Ogunlana, E. O. and Ramstad, E. (1975). Investigation into the antimicrobial activities of local plants. Planta Medica, 27:354-360
Ogunyemi, A.O. (1979). The origin of the herbal cure and its spread. In: Proceedings of a Conference on African Medicinal Plants.
Sofowora, A. (Ed) University of Ife press. 20-22.
Postmes, TJ, Bosch MMC, Dutrieux R, van Baare J, Hoekstra MJ. (1997). Speeding up the healing of burns with honey. An experimental study with histological assessment of wound biopsies. In: Mizrahi A, Lensky Y, editors. Bee Products: Properties, Applications and Apitherapy. Plenum Press, New York.
Rios J. L., Recio M. C., Villar, A. (1988). Screening methods for natural products with antimicrobial activity: A review of the literature. J. Ethnopharmacol. 23: 127-149.
Seymour F. I, and West K S. (1951). Honey - its role in medicine. Med Times 79: 104-7.
Somerfield, S. D. Honey and healing. J R Soc Med 1991; 84(3): 179.
Trease, C. E. and Evans, W. G. (1989). Pharmacognosy, 13th edn., Balliere Tindall, London. 315-679.
Tovey, F. I. (1991). Honey and healing. J R Soc Med 84(7): 447
Waterman, P.G. and Hussain, R.A. (1984) Major xanthrones from Garcinia quadrifaria and Garcinia standii stem bark. Phytochemistry, 21(8): 2099-2101.
Waterman, P. G. and Hussain, R. A. (1985). Lactones, flavonoids and benzophenones from Garcinia conrauana and Garcinia manii. Phytochemistry, 21(6): 1393-1396.