Aspects of Garcina kola Toxicity to a Wild Strain of Saccharomyces cerevisiae from a Brewery Environment
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Abstract
Bitter cola (Garcinia kola) powder and its ethanolic extract were assessed for their ability to inhibit the growth of a wild strain of Saccharomyces cerevisiae isolated from a brewery environment. The iso-alpha acid contents of the G. kola powder, G. kola extract and hop extract were determined as 5.85 mg/l, 6.34 mg/l and 7.65 mg/l, respectively, showing that the iso-alpha acid contents of G. kola extract and G. kola powder compared relatively well with that of hop extract. The antimicrobial activity of both agents, using Humulus lupulus ethanolic extract as control, showed a high dependence on the temperature of assay. At most of the temperatures evaluated (30 – 45 o C), the rates of antimicrobial activity differed. Garcinia kola ethanolic extract and hops extract displayed total inhibition of the wild yeast growth at temperature of 40 o C at concentrations of about 0.039 mg/ml and 0.078 mg/ml respectively. Humulus lupulus extract
(hops) had the highest generation time at 450 C at a concentration of 0.078 mg/ml. The complete inhibition of the yeast growth by G. kola extract at 40 o C indicated that it had higher antimicrobial activity against this yeast than hops extract. In all the temperatures evaluated both G. kola ethanolic extract and powder compared favourably with hops extract with respect to antimicrobial activity against a wild strain of Saccharomyces cerevisiae at various concentrations. These results revealed that the antimicrobial activity of these hopping agents were temperature and concentration dependent.
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References
Adegboye, M. F., Akinpelu, D. A. and Okoh, A. (2008). The bioactive and phytochemical properties of Garcinia kola (Heckel) seed extract on some pathogens. African Journal of Biotechnology, 7(21): 3934-3938
Aina, J. O. and Uko, E. E. (1991). Preliminary studies on tropical hop substitutes. Tropical Science, 31(4): 379 - 385.
Akerele, J. O, Obasuyi, O., Ebomoyi, M. I. Oboh, I. E. Uwumarongie, O. H. (2008). Antimicrobial activity of the ethanol extract and fractions of the seeds of Garcinia kola Heckel (Guttiferae). African Journal of Biotechnology, 7 (2): 169-172. Fig. 7: Re lative effe ct of hopping
age nts on Ye ast ge ne ration time at 45 o C 0 100 200 300 400 500 600 700
0 .0 4 0 .0 8 0 .0 2 0 .0 3 0 .0 6 1.2 5 2 .5 6 5 10 Le vel of hopping agent (mg/ml) Relative effect on Yeast growth GKP HO PS GKE American Society of Brewing Chemist (ASBC)
(1976). Methods of Analysis. 7th edition. Washington D.C. pp 560-562.
Aniche, G. N. and Uwakwe, G. U. (1990). Potential use of Garcina kola as hop substitute in beer brewing. World J. of Micro. and Biotech. 6(3): 323 - 327.
AOAC (2000) Official Methods of Analysis, 17th Edition Vol II Chapter 27, p. 16.
Dosunmu, M. I. and Johnson, E. C. (1995). Chemical evaluation of the nutritive value and changes in ascorbic acid content during storage of the fruit of ‘bitter kola’ (Garcinia kola). Food Chem., 54, 67–71.
Eleyinmi, A. F. and Oloyo, R. A. (2001). Pilot scale brewing trials using formulated blends of selected local vegetables as hop substitutes. J. Food Sci. Technol., 38, 609–611.
Eleyinmi, A. F., Amoo, I. A., Oshodi, A. A. and Adeniran, H. (2004). Evaluation of the hopping potentials of blends of Vernonia amygdalina, Garcinia kola and Gongronema latifolium on sorghum lager beer quality and acceptability. Technical Quarterly of the Master Brewers’ Association of the Americas, 41: 403–407.
Eleyinmi, A. F., Adebowale, Y. A., Oluwalana, I. B., Ajisafe, O. J. and Akintomide, T. F. (2006). Effect of dietary inclusion of Garcinia kola, Gongronema latifolium and Vernonia amygdalina on the nutritional quality of a complementary diet. Research Journal of Biological Sciences, 1(1-4): 43-49.
Ezeifeka, G. O., Orji, M. U., Mbata, T. I. and Patrick, A.O. (2004). Antimicrobial activities of Cajanus cajan, Garcinia kola and Xylopia aethiopica on pathogenic microorganisms. Biotechnology, 3(1): 41-43.
Farombi, E. O. (2003). African indigenous plants with chemotherapeutic potentials and biotechnological approach to the production of bioactive prophylactic agents. African J. Biotech. 2: 662–671.
Federal Office of Statistics (FOS) (1986). The Nigerian trade summary. Federal Ministry of Commerce and Industry Abuja Nigeria.
Fernandez, J. I. and Simpson, W. Y. (1993). Aspects of resistance of Lactic acid bacteria to Hop bitter acids. Journal of Applied Bacteriology, 75: 315 – 319.
Gentalium, A. (1975). Some common medicinal and poisonous plants used in Ethiopian folk medicine in: Useful plants of West Tropical Africa. Vol. 1, 2nd edition (edited by Hutchinson K J, Dalziel M J) Royal Botanical gardens, Kew, London pp. 503-508.
Hough, J. S. (1986). Hops and wort treatment in: Introduction to Brewing Science and Technology Part 1 (eds Rainbow C, Fleat GES). The Inst. Brew. London pp. 28-41.
Iwu, M. M. and Okolo, B. N (1993). Evaluation of Garcina kola seeds as Hop substitute in lager beer brewing. Beer Section/Group Research Project 1 – 21.
Monago, C.C. and Akhidue, V. (2002). Estimation of tannin, saponin, oxalate, cyanogenic and cardiac glycosides in Garsinia kola. Journal of Applied Sciences and Environmental Management, 6(1): 22-25.
Ogu, E. O. and Agu, C. R. (1995). A comparison of some chemical properties of Garcinia kola and Hops for assessment of Garcinia Brewing value. Bioresource Technol., 54: 1–4.
Okafor, N. and Anichie, G. N. (1983). West African Hop substitutes for sorghum lager beer. Brew. Dist. Int. 13(1): 20-23, 31.
Okoro, C. E. (1993). Development of Hop substitutes from Tropical Plants. NBTE J. Agric. Technolo. Maiden ed. p. 30-35.
Okoro, C. C. and Aina, J. O. (2007). Effect of storage on the brewing properties of tropical hop substitutes. African Journal of Biotechnology, 6(12):1479-1483.
Okunji, C.O., Ware, T. A., Hicks, R. P., Iwu, M. M. and Skanchy, D. J. (2002). Capillary electrophoresis determination of biflavanones from Garcinia kola in three traditional African medicinal formulations. Planta Med., 68: 440–444.