The “Journal of Biological Research and Biotechnology (Bio-Research)” is a peer-reviewed, multidisciplinary, international, scientific Open Access Journal that provides publication of articles on biological sciences and biotechnology. The journal established in 2003, is published by the faculty of Biological Sciences, University of Nigeria, Nsukka, Nigeria. The Journal welcomes submission of manuscripts in the form of original and reviews articles, brief and case reports, special communications and editorials, that meet the general criteria of significance and scientific excellence. Papers will be published online approximately one-to-two weeks after acceptance.

Publication of articles in the Journal of Biological Research and Biotechnology (Bio-Research) involves several parties, each of which performs an essential role in achieving the aims and objectives of the journal. Thus, all players (author, the journal editor, the peer-reviewer, and the publisher) are expected to meet and uphold standard norms of ethical behaviour from submission to the publication stage, depending on the area of involvement.

Current Issue

Vol. 24 No. 2 (2026)

Published: 2026-08-31

Articles

Phytochemical compositions of gooseberry, tamarind, soursop and Jack fruit

Evaluation of Nutrient and Phytochemical Composition of Selected Fruits and Health Implications to People in Kampala Capital City Authority, Uganda

Asio Santa Maria, Stephen Kigozi, Mwamini Mwamini (Author)

518-521

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Histological observation of wounds on Day 6 and Day 12. A = Gel/KOH; B= Gel/KOH l; C= Gel-/R1; D= Gel-/R1 ; E=L Mesitran; F=L Mesitran ; G= Gel+/R1; H= Gel+/KR1 Nv = Neovascularization; Hf = Hair follicle; Fb = Fibroblast; U= Ulceration; E = Squamous epithelium; Ci=inflammators cells. Hematoxylineosin (H&E) staining. Olympus BX 51 light microscope. Scale bar is 10 μm.

Comparative Investigation on Wound Healing Potential of Gel Diffusion and Liquid Application of Adansonia digitata Active MoleculesComparative Investigation on Wound Healing Potential of Gel Diffusion and Liquid Application of Adansonia digitata Active Molecul

GAFFO Abdou Rachid, Halida SIBABI, AKPALO Amivi Edéfia, Tchin Darre, Kossi Metowogo, Kwashie EKLU-GADEGBEKU (Author)

522-530

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Mechanisms of environmental factors-induced activation and motility kinetics in fish spermatozoa.

A Systematic Review of Sperm Competition in Fish Under Biological and Ecohydrological Influence

Uduak Jacob, Olusola Akangbe, Emmanuel D. Omogbemi (Author)

531-539

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Synthesis and Preliminary Physicochemical Characterization of Medicinal Plant Extract-Loaded Hyaluronic Acid–Chitosan Nanoparticles for Cancer Drug Delivery

Synthesis and Preliminary Physicochemical Characterization of Medicinal Plant Extract-Loaded Hyaluronic Acid–Chitosan Nanoparticles for Cancer Drug Delivery

Odimegwu Joy Ifunanya, San Millán Rodríguez Irene, González Velázquez, Viviana Jehová (Author)

540-549

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Heavy metal concentrations in soils and edible plants collected from selected dumpsite associated locations within Benin City metropolis. Samples collected during each monthly visit were analysed separately. For each station, heavy metal concentrations obtained from the three-monthly sampling events were averaged to obtain a single mean concentration value. Consequently, the values reported in this study represent the mean ± standard error of measurements obtained over the three-month sampling period

Heavy Metal Contamination, its Transfer from Dumpsite Soils to Vegetables and its Risk to Human Health in Benin City, Nigeriaa

Imelda Iyorah-Elliot, Ayooluwa O. Olayinka (Author)

550-557

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Amino acid alignment showing the effect of potential SNPs on amino acid sequence along the sequenced PTEN gene

Molecular Characterization of PTEN Gene Alterations in Men with Prostate Cancer in Osun State

Dr. Ebenezer Olubunmi ESAN, AYODEJI AJILEYE, Prof. Frederick Olusegun AKINBO (Author)

558-564

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Environmental pathways of endocrine disrupting chemicals.

Endocrine Disrupting Chemicals and their Ecological Effects on Reproduction and Development: A Review

Ibeh Gabriel Ifeoma, Chinwe C. Isitua (Author)

565-574

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Antimicrobial susceptibility of Salmonella isolates obtained from poultry samples

Phenotypic Antimicrobial Resistance and Resistance Genes in Salmonella isolates from poultry farms in Umuahia, Abia State, Nigeria

Emmanuel Enya, Mgbemena, C.E. , Prof. Ekundayo, E.O. (Author)

575-580

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Antibiotic susceptibility patterns of Staphylococcus isolates

Antibacterial Susceptibility Patterns of Staphylococcus species to Common Antimicrobial Agents and Impact on Patients in Bayelsa State, Nigeritate, Southern Nigeria

Theophilus Ayibanoah, Suleiman A. Ismail, *Olatunji K. Aremu, Oluwatoyosi O. Moradeke, and Dengiyefa B. Otuogha (Author)

581-584

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Corrosion rates of galvanised and mild steel in different water samples

Influence of Chemolithotrophic Bacteria on Corrosion Rates of Galvanised and Mild Steel in Sea and Tap Water

Kamoru Tunde Aliu, Olayide F. Obidi , Hafiz A. Akinyele, Cheryl I. Nwokeoma , Adedoyin E. Ayodele, Olawale P. Odeleye, Hanson O. Segun (Author)

585-584

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Graphical representation of the number of bacterial isolates associated with Various hospital location

Antimicrobial Resistance Profile of Multi-Drug Resistant Gram-Negative Bacteria Associated with Hospital Wastewater in Niger State, Nigeria

Uche M. Okoye, Austin Sunday (Author)

594-597

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Spatial Dispersion of Spent Engine Oil in Soil and Gradient-Dependent Effects on Growth and Physiological Responses of Spinach

Spatial Dispersion of Spent Engine Oil in Soil and Gradient-Dependent Effects on Growth and Physiological Responses of Spinach (Spinacia oleracea L.)

Musa Saheed Ibrahim, Chinwe Christy Isitua, and Thelma Konyeme (Author)

598-604

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3D and 2D Sitosterol-6KUW binding site interaction analysis

Phytochemical Profiling and In Silico Evaluation of Antioxidant and Neuropharmacologically Active Constituents of the Methanolic Stem Bark Extract of Magnolia officinalis Using HPLC-UV

Ethelbert Uchechukwu Ezeji , Chiedu A.Okoro, Stella N, Nmezi, Magnus C. Nwoko, Doris I. Ukairo, Raymond C. Ibe (Author)

605-617

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Correlation of Framingham scores, QRISK scores and ASCVD scores with anthropometric measures

Gender Disparities in Cardiometabolic Risk Factors Among Young Nigerian Adults

Augustine C. Onu, Prof. Ifeoma. I. Ijeh, Nwawuba, Nnaemeka Ikenna (Author)

618-624

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PCR amplification of ISSR/SCoT marker loci in H. crinita and cluster analysis of H. crinita samples. Electrophoretic profiles of H. crinita mother (Mo) and in vitro regenerants showing marker bands/loci amplified using (a) UBC 873 (b) SCoT 1 (c) SCoT 10 primers. (d) UPGMA clustering dendrogram showing the relationship between mother H. crinita plant (Mo) and in vitro regenerants. M: Gene ladder wide 2 (0.1 – 20 kbp); Mo: Mother plant; S1 – S5: regenerants derived from shoot explants; H1 – H5: regenerants derived from hypocotyl explants.

Molecular Assessment of Clonal Fidelity in In Vitro-Regenerated Heinsia crinita (African bush apple) Micro Shoots Using ISSR and SCoT markers

Peter Nkachukwu Chukwurah, Glory Michael Okosi, Akuoma Ogechi Mbilitam, Miracle Stephen Ojong, Desmond Oluwatobi Chineke, Daniel Yinusa Haruna, Peace Chidera Okeke (Author)

625-631

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Workflow diagram for isolation and polyphasic characterization of culturable endophytes from tuber crops.

Selective Isolation and Polyphasic Characterization of Culturable Endophytes from Tuber Crops: A Critical Appraisal of Methodologies, Challenges, and Evidence-Based Recommendations

Eze I. Chukwuemeka, Eze N. Chinyere, Joshua E. Parker, Obeta J. Ugwuanyi (Author)

532-638

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CRISPR-Cas systems

Adaptive Roles of CRISPR-Cas Systems in Prokaryotic Immunity and Genome Editing: A Narrative Review

Hadiza Ali Muhammad (Author)

639-649

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Giardia lamblia Infection among Primary School Pupils in Kaduna Metropolis

Prevalence, Risk Factors, and Molecular Analysis of Giardia lamblia Infection among Primary School Pupils in Kaduna Metropolis, Nigeria: A Cross-Sectional Study

Ekwueme E. Ijeoma (Author)

649-659

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Map of Peri Urban Communities, Ibadan, Nigeria.

Groundwater Recharge Dynamics and Sustainability Under Changing Rainfall Patterns in Ibadan Peri-Urban Communities, Nigeria

KUWANDE, Semako B. (Ph.D), , ONAFESO, Olumide D. (Ph.D),, BELLO, Abdulganiy A.(PhD), , SADIKU, Olalekan A. (Author)

660-664

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Effect of traditional processing on the proximate composition of Treculia africana seeds. Values (% dry weight) for raw, cooked, and toasted seeds are from Runsewe-Abiodun et al. (2018), determined by AOAC methods. Crude protein, fat, fibre, and ash all increased with thermal processing.

African Breadfruit (Treculia africana) in sub-Saharan Africa: Nutritional Value, Underutilisation, and Biotechnological Prospects, A Review

Dr Yemisi Olagunju, Dr. Titi Fasuan, Dr Mobolaji Oladunjoye (Author)

665-672

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Applications of AI in Global health solutions for antibiotic resistance

Applications of Artificial Intelligence (AI) in Enhancing Laboratory Procedures and Antimicrobial Resistance Detection

Teslim Shitu, Ruth Bukola Tomilayo, Adedeji Nurudeen Lawal, Bashirat Abdulkareem Saad, Mohammed M. Ahmed, Kafayat Funmi Bamidele, Mulikat Sulaiman Abubakar, Nafisa Ahmad Dandume (Author)

673-681

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Distribution of composite diabetes-like (DM-like) transcriptional scores in CRC. Violin ‎plots showing (A) core and (B) extended composite DM-like scores. Embedded boxplots ‎indicate the median and interquartile range. Both scores show broad continuous distributions, ‎demonstrating inter-tumoural heterogeneity in DM-like transcriptional states.‎

Integrative Transcriptomics Reveals Immune–Metabolic and Signalling Crosstalk in a Diabetes-Like State of Colorectal Cancer

Henry Okuchukwu Ebili (Author)

682-691

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Effect of different concentrations of sodium chloride on growth curve of S. dimorphus mixotrophic culture at 80 µmol m-2s-1 and 25 ºC

Effect of Sodium Chloride Concentrations on Scenedesmus dimorphus (Turpin) Kützing Growth and Fatty Acid Composition Under Mixotrophic Conditions

Kenneth E. Ogbonna, Ughamba T. Kingsley , Joseph Akor, Chijioke Eze, Godwin C. Ugwu, Chiugo C. Aduba et al. (Author)

692-697

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Intensity of soil-transmitted infection among adults at Nsukka Health Center

Prevalence and Intensity of Soil-transmitted Helminths Among Adults Attending Nsukka Health Centre in Nsukka LGA, Enugu State, Nigeria

Dr. Ebele Augustina Orji, Ven. Christian Tochukwu Orji, Dr. Elijah Sunday Okwuonu, Miss Peace Onyinyechi Ugwuele, Dr. Hope Chinwe Ezinwa, Dr. Nelson Ike Ossai, Dr. Ijem Anya Nnachi, Dr. Chinenye Maria-Goretti Ohanu, Dr. Chike Chukwuenyem Ebido, Miss Ozioma Caroline Umennadi (Author)

698-702

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Antibiotics percentage resistance of organisms isolated from Lettuce purchased from Hausa Quarters market in Abakaliki. Ps = Pseudomonas aeruginosa, Sal = Salmonella spp, SHIG = Shigella spp, STAPH = Staphylococcus spp, Proteus =Proteus spp, CIP = ciprofloxacin, AMC = Amoxicillin/clavulanic Acid, W = Trimethoprim, AMP = Ampicillin, IPM = Imipenem, CTX = Cefotaxime , CRO = Ceftriaxone , SXT = Sulfamethoxazole /Trimethoprim, CN = Gentamicin, CAZ=cefltazidime

Bacterial Contamination and Multi-drug Resistance in Lettuce Leaves: A Case Study from Abakiliki Metropolis, Nigeria

Ogochi F. Paul-Igwe et al (Author)

703-708

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Effects of varying concentrations of glycine betaine on the height of garden egg under water-regulated conditions. Difference in lowercase letters on bars in each week indicate significant differences among treatments at p < 0.05.

Glycine Betaine Enhances Growth Markers, Nutritional Quality and Antioxidant Activities in Garden Egg (Solanum aethiopicum L.)  Under regulated Water Conditions

Anthony Ojewumi, Oluwasegun Fawibe, Samuel Ayoola, Sheily Nneka Egonu, Oluwaseun Faith Akinyemi, Kehinde Theophilus Omolokun, abdullahi Adeyemi Adegoke, Racheal Tope-akinyetun , Daniel Adelugba (Author)

709-715

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Dose–Time Mortality Relationships in Cockroach Populations Exposed to Synthetic and Botanical Gel Formulations. Plots : a: fipronil gel b: imidacloprid gel c: Sodom apple based gel d: neem-ginger based gel e: bait matrix only e: peanut butter only he tested dose range.

Comparative Toxicity of Synthetic and Botanical Gel Baits against Periplaneta americana  (Blattodea: Blattidea)

Bolajoko B. Adewoye, Ajoke A. Adeleye, Kalu N. Ezinne , Philip-Attah M. Tejumade, Kayode D. Ileke, Joseph C. Anikwe (Author)

716-723

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Fishing gear types, usage frequency, and community acceptability scores

Assessment of Fishing Gear Acceptability, Capture Efficiency, and Species Diversity Among Artisanal Fishers in River Benue, Taraba State, NigeriaA STATE, NIGERIA

Bonjoru Rifore, Jerry Timothy Jerry, Amadu Samuel Otiki, Luka Kazy (Author)

724-731

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Antibacterial screening of fractions against poultry-associated multidrug-resistant isolates. Maximum-likelihood phylogenetic tree of endophytic bacteria (ENDVI (PX482400.1), and ENDVII (PX446467.1)) Based on 16s rDNA sequence using Iqtree software.

Antibacterial Activity and Metabolite Profile of Endophytic Bacteria from Azadirachta Indica and Khaya Senegalensis against Poultry-Associated Antibiotic-Resistance Escherichia Coli and Salmonella Spp

Ahmad Aliyu Muhammad and Abdullahi Bello (Author)

732-741

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Physicochemical Properties of Thiaminase from Cassava Tubers

Physicochemical Properties of Thiaminase from Cassava Tubers (Manihot esculenta)

Moromoke A. Fadunsin, Kehinde Okanlawon, Aanuoluwa F. Olaitan , Raphael E. Okonji (Author)

742-746

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Representative peripheral blood film morphology of Wistar rats following benzene exposure and selected medicinal plant extract administration (Leishman stain, ×1000 magnification). Group A (Control): Arrows indicate normal erythrocytes, segmented neutrophils, and lymphocytes with preserved morphology. Group B (Benzene-induced haematopoietic dysfunction): Arrows indicate increased lymphocytes, atypical/immature lymphocyte-like cells, and altered leukocyte morphology. Group C (Plant extract-treated): Arrows indicate improved peripheral blood morphology with the presence of segmented neutrophils, lymphocytes, and normal erythrocytes. Group D (5-Fluorouracil-treated): Arrows indicate segmented neutrophils, lymphocytes, and eosinophils showing relatively preserved leukocyte morphology. R (Erythrocyte), N (Segmented neutrophil), L (Lymphocyte) I (Atypical/immature lymphocyte-like cell) and E (Eosinophil).

Modulatory Effects of Selected Medicinal Plant Extracts on Benzene-Induced Haematopoietic Dysfunction in Wistar Rats

Adedeji D. Atere (Author)

747-751

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Effect of the aqueous extract of Mangifera indica leaves

Comparative effect of aqueous extract of mango leaves (Mangifera indica) and glibenclamide® on physiological indices of Wistar Rats induced with type II diabetes

Felix Andong, Ifeoma Nwuzor, Ruth Agyo (Author)

752-759

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Principal component analyses (PCA) indicating the score plot for the first and second components. The smallest oval shape circled by yellow line indicates distribution of variables during the first period of extract administration; the smaller oval shape circled by blue line indicates distribution of variables during second period of extract administration; the larger oval shape circled by pink line indicates distribution of variables during third period of extract administration; the large oval shape circled by red line indicates distribution of variables during estrus period; the larger oval shape circled by green line is the post period of no extract administration. The arrows of the PCA indicates approximation of variance of the variables, and then their angles approximate the correlations. More so, points closer to each other usually signify that they have similar score on PCA components (n = 18 female rats).

Possible Alterations of Changes in Weight, Appetite and Reproductive Biomarkers of Female Wistar Rats Using Blends of Cucumber, Green Tea and Orlistat®Wistar rats

Felix Andong, Ifoema Nwuzor, Ruth Agyo (Author)

760-767

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Rat lungs control show; normal alveoli (NV), bronchial artery (BA), interstitial space (IS) and terminal bronchiole (TB); plate B. Rats lungs given 50mg/kg of caffeine show: normal alveoli (NA), dilated terminal bronchiole (TB) and activated mononuclear phagocyte cell (AM), plate C. Rats lungs given 100mg/kg of caffeine show: normal alveoli (NA), interstitial congestion (VC) and dilated terminal bronchioles (TB), plate D. Rats lungs given 200mg/kg of caffeine show: normal alveoli (NA), interstitial vasodilation (VD) and congestion (VC), activated bronchio-alveolar lymphoid aggregate, H&E 100X

Effect of Therapeutic Caffeine Doses on the Histology of Lung Tissue in Experimental Animal Models

Benson Onyeije, prof Silvanus Olu Innih (Author)

768-772

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Mean ± standard deviation (SD) with observed ranges. TC = Total cholesterol; HDL-C = High-density lipoprotein cholesterol; LDL-C = Low-density lipoprotein cholesterol; VLDL-C = Very-low-density lipoprotein cholesterol; TG = Triglycerides

Highly Active Antiretroviral Therapy alters Serum Lipids and Hepatic Transaminases in HIV-Positive Adults: A Cross-Sectional Studym Enugu State University of Science and Technology Teaching Hospital, Nigeria

Job Popme Ndumbe (Author)

773-777

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UPGMA dendrograms of Lagenaria siceraria accessions based on Gower distance, cut at k = 3. Tip labels are coloured by state of origin: Ondo (blue), Ogun (orange), Osun (green), Oyo (red); branches are coloured by cluster membership. (A) Leaf (24 accessions, 6 informative characters). (B) Stem (22 accessions, 5 characters). (C) Proximal petiole (15 accessions, 6 characters). (D) Distal petiole (20 accessions, 6 characters).

Anatomical variation in leaf, stem and petiole of Lagenaria siceraria from southwestern

Dr Yemisi Olagunju, Prof. A.A. Jayeola, Dr. Olu Matthew, Prof. O.J. Olawuyi (Author)

778-786

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